一测多评法同时测定不同配伍比例枳实-厚朴药对中11种成分的含量  

Simutaneous content determination of eleven constituents in different compatibility ratios of Fructus Aurantii Immaturus-Magnoliae Officinalis Cortex drug pair by QAMS

作　　者：黄婷[1] 柳贤福[1,2] 陈汝旭 陈霞 李明阳 丁聪[2] HUANG Ting;LIU Xian-fu;CHEN Ru-xu;CHEN Xia;LI Ming-yang;DING Cong(Guangxi University of Chinese Medicine,Nanning 530222,China;Faculty of Chinese Medical Science,Guangxi University of Chinese Medicine,Nanning 530222,China)

机构地区：[1]广西中医药大学,广西南宁530222 [2]广西中医药大学赛恩斯新医药学院,广西南宁530222

出　　处：《中成药》2024年第12期3911-3917,共7页Chinese Traditional Patent Medicine

基　　金：国家级大学生创新创业训练计划项目(202213643012);广西壮族自治区卫生健康委自筹科研课题(Z-A20231290);广西高校中青年教师科研基础能力提升项目(2022KY1665,2022KY1673)。

摘　　要：目的建立一测多评法同时测定不同配伍比例枳实-厚朴药对中辛弗林、紫丁香苷、木兰花碱、芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、柠檬苦素、柚皮素、和厚朴酚、厚朴酚的含量。方法枳实、厚朴分别按1∶1、1∶2、2∶1比例配伍。分析采用Agilent 5TC-C_(18)色谱柱(4.6 mm×250 mm,5μm);流动相甲醇-0.1%磷酸,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长280 nm。以橙皮苷峰为内标,计算其他10种成分相对校正因子,测定其含量。结果11种成分在各自范围内线性关系良好(r≥0.9990),平均加样回收率97.04%~99.45%,RSD 1.22%~2.48%,一测多评法所得结果与外标法接近,配伍比例为1∶1各成分含量最高。结论该方法简便准确,重复性好,可用于枳实-厚朴药对质量控制。2种药材配伍比例为1∶1时,更有利于各成分提取。AIM To establish a quantitative analysis of multi-components by single-marker(QAMS)method for the simultaneous content determination of synephrine,syringin,magnoflorine,narirutin,naringoside,hesperridin,neoesperridin,limonin,naringenin,honokiol and magnolol in different compatibility ratios of Fructus Aurantii Immaturus-Magnoliae Officinalis Cortexdrug pair.METHODS The ratios of 1∶1,1∶2 and2∶1 were adopted in the compatibility ofFructus Aurantii ImmaturusandMagnoliae Officinalis Cortex,respectively.The analysis was performed on a 30℃thermostatic Agilent 5TC-C_(18)column(4.6 mm×250 mm,5μm),with the mobile phase comprising of methanol-0.1%phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 280 nm.Hesperidin was used as an internal standard to calculate the relative correction factors of the other ten constituents,after which the content determination was made.RESULTS Eleven constituents showed good linear relationships within their own ranges(r≥0.9990),whose average recoveries were 97.04%-99.45%with the RSDs of 1.22%-2.48%.The result obtained by QAMS approximated those obtained by external standard method.CONCLUSION This simple,accurate and reproducible method can be used for the quality control ofFructus Aurantii Immaturus-Magnoliae Officinalis Cortex drug pair.The extraction of various constituents is more favourable when the compatibility ratio of two medicinal materials is 1∶1.

关 键 词：枳实 厚朴 药对 配伍比例 化学成分 含量测定 一测多评 

分 类 号：R284.1[医药卫生—中药学]