鸦胆子苦木内酯类与木脂素类成分及其抗炎活性研究  

Quassinoids and lignans from Brucea javanica and their antiinflammatory activities

作　　者：栾同同 何亚玲 辛璐 蒙康婷 刘永宏 苏志维[1] LUAN Tong-tong;HE Ya-ling;XIN Lu;MENG Kang-ting;LIU Yong-hong;SU Zhi-wei(Institute of Marine Drugs,Guangxi University of Chinese Medicine/Guangxi Key Laboratory of Marine Medicine,Nanning 530200,Guangxi;Key Laboratory of Tropical Maine Bio-resoures and Ecology(South China Sea Institute of Oceanology,Chinese Academy of Sciences)/Guangdong Key Laboratory of Marine Materia Medica,Guangzhou 510301,Guangdong)

机构地区：[1]广西中医药大学海洋药物研究院/广西海洋药物重点实验室,广西南宁530200 [2]热带海洋生物资源与生态重点实验室(中国科学院南海海洋研究所)/广东省海洋药物重点实验室,广东广州510301

出　　处：《中成药》2024年第12期4035-4041,共7页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金(32060098);广西八桂学者专项经费(05019055);广西中医药大学“桂派杏林青年英才”项目(2022C039);广西中医药大学海洋药物研究院团队科研专项(2018ZD005-A08)。

摘　　要：目的研究鸦胆子Brucea javanica(L.)Merr.苦木内酯类与木脂素类成分及其抗炎活性。方法提取物采用硅胶、MCI、Sephadex LH-20及HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用RAW264.7模型评价其抗炎活性。结果从中分离得到7个苦木内酯类化合物,分别鉴定为bruceanic acid E(1)、15-O-(3-hydroxy-3-methy1butanoyl)-brucealide(2)、bruceine L(3)、aglycone of yadanzioside D(4)、javanicolide A(5)、javanicolide C(6)、bruceanic acid A(7);4个木脂素类化合物,分别鉴定为cleomiscosin A(8)、ceplignan(9)、threoguaiacylglycerol 8′-(4-hydroxymethyl-2-methoxyphenyl)ether(10)、erythro-guaiacylglycerol 8′-(4-hydroxy-methyl-2-methoxyphenyl)ether(11)。化合物3、8可抑制RAW264.7细胞产生NO。结论化合物9~11为首次从鸦胆子属植物中分离得到。化合物3、8具有抗炎活性。AIM To study the quassinoids and lignans fromBrucea javanica( L.) Merr.and their antiinflammatory activities.METHODS The extract was isolated and purified by silica gel,MCI,Sephadex LH-20and HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Seven quassinoids were isolated and identified as bruceanic acid E(1),15-O-(3-hydroxy-3-methy1butanoyl)-brucealide(2),bruceine L(3),aglycone of yadanzioside D(4),javanicolide A(5),javanicolide C(6),bruceanic acid A(7).Four lignans were isolated and identified as cleomiscosin A(8),ceplignan(9),threo-guaiacylglycerol 8′-( 4-hydroxymethyl-2-methoxyphenyl ) ether(10),erythroguaiacylglycerol 8′-(4-hydroxy-methyl-2-methoxyphenyl) ether(11).Compounds3,8could inhibit NO release in RAW264.7 cells.CONCLUSION Compounds9-11are isolated fromBruceagenus for the first time.Compounds3and8have anti-inflammatory activities.

关 键 词：鸦胆子 苦木内酯 木脂素 分离鉴定 抗炎活性 

分 类 号：R284.1[医药卫生—中药学]