中国蓝藻新记录种--纤细双色藻(Cyanobium gracile)  

A newly-recorded cyanobacterial species Cyanobium gracile in China

作　　者：刘洋 委雅菲[1,2] 李效宇 张榜军 Liu Yang;Wei Yafei;Li Xiaoyu;Zhang Bangjun(Henan International Joint Laboratory of Aquatic Ecotoxicology and Health Protection,Xinxiang 453007,P.R.China;College of Life Sciences,Henan Normal University,Xinxiang 453007,P.R.China;Observation and Research Station on Water Ecosystem in Danjiangkou Reservoir of Henan Province,Nanyang 474450,P.R.China)

机构地区：[1]河南省水生态毒理与健康防护国际联合实验室,新乡453007 [2]河南师范大学生命科学学院,新乡453007 [3]河南省丹江口水库水域生态系统野外科学观测研究站,南阳474450

出　　处：《湖泊科学》2025年第1期229-237,共9页Journal of Lake Sciences

基　　金：国家重点研发计划项目(2021YFE0112000);河南省国际科技合作项目(232102521014)联合资助。

摘　　要：随着基因测序技术的发展,环境中一些微藻不断被发现,超微蓝藻便是其中之一,但关于超微蓝藻的多相分类学研究在我国报道较少。本研究首次在某自来水厂分离纯化获得了一株超微蓝藻藻株Cyanobium gracile HNU001,并对其形态学、超微结构和分子特征进行了研究。形态学研究发现,其藻细胞呈椭圆形或椭球形,在分裂过程中平行于短的细胞轴对称存在;超微结构显示了细胞二分裂繁殖以及类囊体膜平行于细胞膜排列;16S rRNA基因序列显示,该藻株与双色藻属Cyanobium的序列相似性大于99.9%,分子系统树聚为一束;该藻株与Cyanobium gracile PCC 6307的D1-D1′、Box-B螺旋结构一致。综合以上结果,将其命名为纤细双色藻(Cyanobium gracile),是在我国的首次报道。With the advancement of gene sequencing technology,researchers have been able to uncover microalgae(e.g.,picocyanobacteria)that were previously unknown in the environment.Despite this,previous publications shed limited light on the comprehensive taxonomic analysis of picocyanobacteria in China.This study focused on the isolation and purification of Cyanobium gracile HNU001 from a waterworks,which was the first study reporting this species in detail.The morphology,ultrastructure,and molecular characteristics of the organism were investigated.Morphological observations showed an ellipsoidal or elongated shape of the algal cells that divided symmetrically into shorter cells.Ultrastructural analysis indicated that cell division through dimitosis and the arrangement of thylakoid membranes were parallel to the cell membrane.Analysis of the 16S rRNA gene sequences demonstrated a high similarity of over 99.9%between this strain and Cyanobium,placing it within a distinct molecular cluster.Comparative analysis of the D1-D1′and Box-B helical structures of Cyanobium gracile HNU001 with Cyanobium gracile PCC 6307 showed high consistency.Based on these findings,the species was formally named Cyanobium gracile,i.e.,the first taxonomic characterization in China.

关 键 词：蓝藻 超微蓝藻 双色藻 分类 分子系统 

分 类 号：Q949.22[生物学—植物学]