罗非鱼饲料绿原酸发酵配比及时长的筛选研究  

Study On Screening of Chlorogenic Acid Fermentation Fatio and Time Length in Tilapia Feed

作　　者：程蔓 朱文虎 古昌辉 韦云勇 樊宪伟 麻艳群 CHENG Man;ZHU Wenhu;GU Changhui;WEI Yunyong;FAN Xianwei;MA Yanqun(College of Animal Science and Technology,Guangxi University/Key Laboratory of Healthy Aquaculture and Nutritional Regulation of Aquatic Organisms in Guangxi Universities,Nanning,Guangxi 530004,China;School of Life Science and Technology,Guangxi University/State Key Laboratory for Protection and Utilization of Subtropical Agricultural Biological Resources,Nanning,Guangxi 530004,China;Guangxi LvYan Biotechnology Co.,Ltd,Nanning,Guangxi 530100,China)

机构地区：[1]广西大学动物科学技术学院,广西高校水生生物健康养殖与营养调控重点实验室,广西南宁530004 [2]广西大学生命科学与技术学院,亚热带农业生物资源保护与利用国家重点实验室,广西南宁530004 [3]广西绿原生物科技有限责任公司,广西南宁530100

出　　处：《水产养殖》2024年第12期6-11,共6页Journal of Aquaculture

基　　金：广西重点研发计划(桂科AB21238005);南宁市“邕江计划”领军人才团队专项(2024017)。

摘　　要：在罗非鱼商品饲料中添加枯草芽孢杆菌菌液,利用枯草芽孢杆菌发酵饲料,测定不同配比及时长下发酵饲料中绿原酸的含量。设置5个试验组,分别为A、B、C、D和E组。对应枯草芽孢杆菌液体积(mL)和商品饲料质量(g)的比例为1∶2、1∶3、1∶4、1∶5,不添加枯草芽孢杆菌液的饲料为E组,饲料发酵时长分别为6,12,18,24,30,36,42和48 h。采用高效液相色谱法测定饲料中绿原酸的含量。结果表明,42 h时,各组饲料中绿原酸含量均最高(P<0.05),6 h时均最低。C组和D组绿原酸含量变化趋势基本一致,且各发酵时长绿原酸含量均显著高于A组和B组(P<0.05)。A、B、C、D组绿原酸含量随发酵时间增加总体呈“升→降→升→降”的趋势。双因素分析结果表明,配比浓度(因素A)、发酵时长(因素B)及该双因素交互作用(因素A×因素B),均对绿原酸产量的合成有极显著影响(P<0.01);根据各因素F值大小,各因素影响的主次顺序为:因素A、因素B、因素A×因素B。指出,枯草芽孢杆菌液和饲料的最佳配比为1∶5,最佳的发酵时长为42 h。Bacillus subtilis bacterial liquid was added into tilapia commercial feed,and then the the content of chlorogenic acid was dected at different ratios and durations after being fermented in the feed.There were five experimental groups set up,and the ratio of the volume of Bacillus subtilis liquid(mL)to the mass of commercial feed(g)were 1∶2(Group A),1∶3(Group B),1∶4(Group C),1∶5(Group D).The feed was Group E without Bacillus subtilis liquid,and the fermentation time of the feed were 6,12,18,24,30,36,42,and 48 hours,respectively.Finally high performance liquid chromatography was used to determine the content of chlorogenic acid in the feed.The results showed that the content of chlorogenic acid in all groups of feed was highest at 42 hours(P<0.05),and lowest at 6 hours.The trend of chlorogenic acid content changes in Group C and Group D were basically consistent.And the chlorogenic acid content in Group C and Group D were significantly higher than those in Group A and Group B at all fermentation times(P<0.05).With fermentation time going by,the content of chlorogenic acid in groups A,B,C,and D showed an overall trend of“increase→decrease→increase→decrease”.The results of the two factor analysis showed that the single factor of ratio concentration(A),fermentation duration(B),and the interaction of these two factors(A×B)all had significant impacts on the synthesis of chlorogenic acid yield(P<0.01).According to the magnitude of each factor’s F-value,the order of priority for each factor was:A,B,A×B.The optimal ratio of Bacillus subtilis addition to feed was 1∶5,and the optimum fermentation duration was 42 hours.

关 键 词：绿原酸 枯草芽孢杆菌 发酵饲料 罗非鱼 

分 类 号：S963[农业科学—水产养殖]