Targeted Analysis of VOCS in Exhaled Breath of Coal Workers’ Pneumoconiosis Patients, An Exploratory Study  

作　　者：Rui Gao Lijia Yu Qingyu Meng Yazhen He Fangda Peng Xiangfu Cao Qingjun Qian Qicai Liu Gaisheng Liu Fan Yang Yiwei Shi Qingsong Chen Chunguang Ding 

机构地区：[1]National Center for Occupational Safety and Health,NHC,Beijing,China [2]NHC Key Laboratory for Engineering Control of Dust Hazard,Beijing,China [3]Guangdong Pharmaceutical University,Guangzhou City,Guangdong Province,China [4]Xishan Coal and Electricity(Group)Co.,Ltd.Occupational Disease Prevention and Control Center,Taiyuan City,Shanxi Province,China [5]NHC Key Laboratory of Pneumoconiosis,MOE Key Laboratory of Coal Environmental Pathogenicity and Prevention,Shanxi Key Laboratory of Respiratory Diseases,The First Hospital of Shanxi Medical University,Taiyuan City,Shanxi Province,China

出　　处：《China CDC weekly》2024年第52期1403-1409,I0001-I0003,共10页中国疾病预防控制中心周报（英文）

基　　金：Supported by the National Center for Occupational Safety and Health Self-management Project of China(2019009)and the Central Public-Interest Scientific Institution Basal Research Project of China(2020-PT320-005).

摘　　要：Introduction:Pneumoconiosis represents the most prevalent occupational disease in China,with coal workers’pneumoconiosis(CWP)showing the highest incidence.Analysis of volatile organic compounds(VOCs)in the exhaled breath of CWP patients may provide novel insights into its pathogenesis.Methods:Study data were collected through questionnaires and medical examinations.Thermal desorption-gas chromatography-mass spectrometry was employed for targeted VOC analysis.Differential VOCs were identified using OPLS-DA,the Mann-Whitney U test,and fold change analysis.The discriminatory efficacy of differential VOCs was evaluated using receiver operating characteristic(ROC)curves.Spearman correlation analysis explored relationships between differential VOCs,lung function indices,and blood cell levels.Results:The pneumoconiosis group showed elevated concentrations of 10 compounds,including isopentane,n-pentane,and isoprene,while four compounds,including 2,4-dimethylpentane,methylcyclohexane,2,3,4-trimethylpentane,and 2-methylheptane showed decreased concentrations.Combined univariate and multivariate statistical analyses identified six significant VOCs,including isopentane and pentane.Notably,isopentane and npentane demonstrated negative correlations with forced vital capacity and levels,while 2-methylheptane showed positive correlations.Discussion:Clear metabolic differences in VOCs exist between CWP patients and non-dust-exposed healthy controls.Six compounds—isopentane,npentane,3-methylpentane,n-hexane,cyclohexane,and 2-methylheptane—in exhaled breath demonstrate potential as biomarkers for CWP.

关 键 词：ANALYSIS ELEVATED indices 

分 类 号：R135[医药卫生—劳动卫生]