The Maize WRKY Transcription Factor ZmWRKY25 Respond Drought Stress in Transgenic Tobacco  

作　　者：Jianbo Fei Zhibo Liu Piwu Wang Jing Qu Siyan Liu Shuyan Guan Yiyong Ma 

机构地区：[1]Joint International Research Laboratory of Modern Agricultural Technology,Ministry of Education,Jilin Agricultural University,Changchun,130118,China [2]College of Agronomy,Jilin Agriculture Science and Technology College,Jilin,132101,China [3]College of Agronomy,University of Jilin Agricultural,Changchun,130118,China [4]Agricultural Biotechnology Research Institute,Jilin Academy of Agricultural Sciences(Northeast Innovation Center for Agricultural Science and Technology in China),Changchun,130033,China

出　　处：《Phyton-International Journal of Experimental Botany》2024年第12期3617-3635,共19页国际实验植物学杂志（英文）

基　　金：the International Cooperation Joint Laboratory of the Ministry of Educationon Modern Agricultural Technology(H20230034);Jilin Province Science and Technology Development Plan Project(20230202003NC,20230508005RC,20240601060RC).

摘　　要：The WRKY transcription factors play important roles in various biological processes such as plant development,defense regulation,and stress response.Despite this,there is limited information available on drought-related WRKY genes in maize.Through RNA sequencing(RNA-seq)analysis,a WRKY transcription factor called ZmWRKY25 was identified in this study.ZmWRKY25 belongs to Group II and is localized in the nucleus.Protein interaction analysis revealed that ZmWRKY25 interacts with several proteins involved in the abscisic acid(ABA)signal pathway.Expression of ZmWRKY25 was found to be up-regulated in response to drought,salt,and ABA treatments in tobacco plants.Furthermore,over-expression of ZmWRKY25 in tobacco led to improved tolerance to drought by participating in the ABA signal pathway,regulating the reactive oxygen species(ROS)active oxygen scavenging system,and promoting root growth and development.These findings suggest that ZmWRKY25 plays a role in multiple abiotic stress response pathways and acts as a positive factor under drought stress.Overall,the study highlights the importance of ZmWRKY25 in enhancing plant resilience to drought through its involvement in key physiological pathways.

关 键 词：WRKY ZmWRKY25 ABA drought tolerance MAIZE 

分 类 号：Q94[生物学—植物学]