破布叶组培快繁技术  

Tissue culture and rapid propagation technology of Microcos paniculata L.

作　　者：蔡清梅 陈冬怡 陈国华[2] 胡国弟 周宏英 CAI Qingmei;CHEN Dongyi;CHEN Guohua;HU Guodi;ZHOU Hongying(Guangdong Huidakang Pharmaceutical Co.,Ltd.,Foshan Guangdong 528244,China;South China Botanical Garden,Chinese Academy of Sciences,Guangzhou Guangdong 510650,China;Guangdong Liangtian Agriculture and Forestry Technology Co.,Ltd.,Qingyuan Guangdong 511800,China)

机构地区：[1]广东慧达康制药有限公司,广东佛山528244 [2]中国科学院华南植物园,广东广州510650 [3]广东良田农林科技有限公司,广东清远511800

出　　处：《农业工程》2024年第12期45-49,共5页AGRICULTURAL ENGINEERING

基　　金：广东省重点领域研发计划项目(2020B020221002);广州市科技计划项目(2023E04J0007)。

摘　　要：以破布叶茎段为外植体,通过植物组培快繁技术,培养成完整的脱毒植株。试验结果表明,外植体灭菌处理成功率94%,诱导不定芽的最适培养基1/4MS+0.5 mg/L 6-BA;不定芽继代增殖的最适培养基1/4MS+0.5 mg/L 6-BA+0.1 mg/L NAA+0.1 mg/L GA3,增殖系数3.3;最适出根培养基1/4MS+1.0 mg/L IBA+0.1 mg/L NAA,出根率93.1%;组培苗移栽成活率98%,为破布叶种苗提供了一种新型快速的繁殖方式,将促进岭南特色中药材破布叶高效新型产业化的形成和发展。Using stems of Microcos paniculata L.seedlings as explants,complete and virus-free plants were obtained by tissue culture and rapid propagation technology.Results showed that explants sterilization success rate was as high as 94%.Optimal medium for adventitious buds induction was 1/4MS+0.5 mg/L 6-BA.Optimal medium for subculture multiplication was 1/4MS+0.5 mg/L 6-BA+0.1 mg/L NAA+0.1 mg/L GA3,and subculture multiplication factor was up to 3.3.Optimal medium for root was 1/4MS+1.0 mg/L IBA+0.1 mg/L NAA,while rooting rate was 93.1%.Transplanting survival rate was averaged at 98%.It could provide an effect way for rapid propagation of virus-free seedlings,which would promote formation and development of high efficiency and new industrialization of Microcos paniculata L.,a characteristic Lingnan medicinal plant.

关 键 词：破布叶 组培快繁 种苗 中药材 

分 类 号：S33[农业科学—作物遗传育种]