荧光免疫分析仪的光谱成像系统设计  

Design of Spectral Imaging System for Fluorescence Immune Analyzer

作　　者：侯明旺 张光伟[1] 向阳[1] HOU Mingwang;ZHANG Guangwei;XIANG Yang(School of Opto-Electronic Engineering,Changchun University of Science and Technology,Changchun 130022)

机构地区：[1]长春理工大学光电工程学院,长春130022

出　　处：《长春理工大学学报（自然科学版）》2024年第6期56-61,共6页Journal of Changchun University of Science and Technology(Natural Science Edition)

基　　金：吉林省科技发展计划重点研发项目(20220201097GX)。

摘　　要：针对荧光免疫分析仪对光谱成像系统小体积、高分辨率和多通道的需求,提出了使用反射式平面光栅作为分光元件的光谱成像系统。根据反射式平面光栅的分光原理,采用离轴反射式结构与非球面校正透镜组合的方式,实现了对待检光源的精准分光。通过分辨率、光谱范围等设计要求确定了光谱仪的初始结构,利用Zemax软件对结构进行了进一步的仿真优化。仿真结果表明,该光学系统能够在狭缝宽度25μm,光栅刻线0.3的条件下,实现了在多谱段范围内,结构紧凑、分辨率高的设计目标,波长分辨率优于1.5 nm,为荧光免疫分析仪的便携化提供了一种有效的设计方案。In order to meet the requirements of small volume,high resolution and multi-channel spectral imaging system for fluorescence immune analyzer,a spectral imaging system using reflective planar grating as the beam splitting element was proposed.According to the beam splitting principle of reflective grating,the combination of off-axis reflective structure and aspherical correction lens is used to realize the precise beam splitting of the light source to be examined.In this paper,the initial structure of the spectrometer was determined by the design requirements such as resolution and spectral range,and the structure was further simulated and optimized by using Zemax software.The simulation results show that the optical system can achieve the design goals of compact structure and high resolution in the multi-spectral range with a slit width of 25μm and a grating profile of 0.3.The wavelength resolution is better than 1.5nm,which provides an effective design scheme for the portability of fluorescence immune analyzer.

关 键 词：光谱成像系统 荧光免疫分析仪 Czerny-Turner结构 光学系统设计 

分 类 号：O436[机械工程—光学工程]