基于转录组数据的炮仗花SSR序列特征分析  

Analysis of SSR Sequence Characteristics for Pyrostegia venusta Based on Transcriptome Data

作　　者：朱飞雪[1] 程玉江 郭丽[1] 孙利娜[3] 寇艳玲[1] ZHU Feixue;CHENG Yujiang;GUO Li;SUN Li'na;KOU Yanling(He'nan Vocational College of Agriculture,Zhengzhou 451450,China;He'nan Dongbao Greening Engineering Landscape Co.,Ltd.,Zhengzhou 450000,China;Guangxi Academy of Forestry Sciences,Nanning 530002,China)

机构地区：[1]河南农业职业学院,郑州451450 [2]河南东宝绿化工程园林有限公司,郑州450000 [3]广西壮族自治区林业科学研究院,南宁530002

出　　处：《种子》2024年第12期128-132,137,共6页Seed

基　　金：河南省科技厅科技攻关项目“切花芍药设施栽培品种选育及配套栽培技术集成研究”(232102110263);河南农业职业学院“花卉生产与应用科研创新团队”(HNACKT-2023-03);河南农业职业学院“特色花卉植物资源开发与应用科研创新团队”(ZZ2405TD01)。

摘　　要：为了解炮仗花SSR位点分布特征,本研究对炮仗花转录组SSR位点进行挖掘。结果表明,从炮仗花转录组测序数据中获得85 699条非冗余Unigenes,共搜索到22 864个SSR位点分布于18 282条Unigenes上,SSR位点的出现频率为26.68%,平均每4.38 kb出现1个SSR,平均长度为13.89 bp。炮仗花SSR位点主要重复类型为单核苷酸重复(13 780个,占比60.27%),其次为二核苷酸重复(6 141个,占比26.86%)和三核苷酸重复(2 656个,占比11.62%)。在72种重复基元中,单核苷酸A/T基元为最优势基元(12 731个,占比55.68%),其次为二核苷酸优势基元AG/CT(2 522个,占比11.03%)和三核苷酸优势基元AAG/CTT(745个,占比3.26%)。炮仗花SSR位点以5~15次重复为主,6种核苷酸重复类型数量总体表现为随着基元重复次数的增加而减少。SSR位点主要集中在10~18 bp之间,此范围内的SSR数量占SSR位点总数的81.87%,具有较高多态性潜能的SSR(长度>20 bp)位点数为640个,占比10.17%。总之,炮仗花SSR位点出现频率高,SSR位点丰富,具有较高的多态性潜能。In this study,the transcriptome SSR loci of Pyrostegia venusta were excavated in order to understand the distribution characteristics of SSR loci and lay a foundation for the development of SSR markers.The results showed that 85699 Unigenes were obtained from the transcriptome sequencing data of Pyrostegia venusta,and a total of 22864 SSR loci were found on 18282 Unigenes.The occurrence frequency of SSR loci was 26.68%,1 SSR locus every 4.38 kb with an average length of 13.89 bp.The main types of repeats for SSR loci of Pyrostegia venusta were single nucleotide repeats(13780,accounting for 60.27%),followed by dinucleotide repeats(6141,accounting for 26.86%)and trinucleotide repeats(2656,accounting for 11.62%).Among the 72 repetitive primitives,the single nucleotide A/T was the most dominant(12731,accounting for 55.68%),followed by dinucleotide dominant AG/CT(2522,accounting for 11.03%)and trinucleotide dominant AAG/CTT(745,accounting for 3.26%).The number of repeats for SSR loci of Pyrostegia venusta was mainly from 5 to 15 times,of which 6 nucleotide repeats decreased with the increase of the number of primitive repeats.The SSR loci mainly focused from 10 to 18 bp,within which the number of SSR loci in this range accounted for 81.87%of the total number of SSR loci.The number of SSR loci with high polymorphism potential(length>20 bp)was 640,accounting for 10.17%.In conclusion,the frequency of SSR loci of Pyrostegia venusta was high,the SSR loci were abundant,and the polymorphism potential was high.

关 键 词：炮仗花 转录组 SSR 多态性 

分 类 号：S685.99[农业科学—观赏园艺]