藏产卷丹百合鳞茎离体快繁的研究  

Study on Rapid Propagation in Vitro of Lilium lancifolium Bulbs from Xizang

作　　者：尹秀 王丹[1] 王园园 臧瑞玲 禄亚洲 兰小中 YIN Xiu;WANG Dan;WANG Yuanyuan;ZANG Ruiling;LU Yazhou;LAN Xiaozhong(Food Science College,Xizang Agricultural and Animal Husbandry University,Linzhi Xizang 860000,China;Key Laboratory of Tibetan Medicine Resources Conservation and Utilization of Xizang Autonomous Region,Xizang Agriculture and Animal Husbandry University,Linzhi Xizang 860000,China;The Provincial and Ministerial Co-founded Collaborative Innovation Center for R&D in Xizang Characteristic Agricultural and Animal Husbandry Resources,Xizang Agriculture and Animal Husbandry University,Linzhi Xizang 860000,China)

机构地区：[1]西藏农牧学院食品科学学院,西藏林芝860000 [2]西藏农牧学院西藏自治区藏药资源保护与利用重点实验室,西藏林芝860000 [3]西藏农牧学院西藏特色农牧资源研发省部共建协同创新中心,西藏林芝860000

出　　处：《种子》2024年第12期149-156,共8页Seed

基　　金：西藏自治区2021年度重大专项科技计划-藏产百合人工种植技术研究与产业化应用(XZ202101ZD0023G);西藏自治区本级本门预算项目(2019-44);第四次全国中药资源普查西藏自治区中(藏)药资源普查(20200501);中央财政支持地方高校改革发展资金-生物技术一流专业建设项目(2024-007)。

摘　　要：本研究以藏产卷丹百合的地下鳞茎为实验材料,采用不同接种方式、不同部位的鳞片及不同生长年限的鳞片,探索繁殖系数较高的离体快繁体系。结果表明,采用卷丹百合内层鳞片的芽诱导效果较好,且污染率较低,最佳接种方式为近轴面朝下,可显著地提高芽诱导数量,纵划处理有利于鳞片的芽诱导,三年生鳞片的诱导效果较好,所诱导的不定芽更加粗壮,长势好。最佳的鳞片诱导培养基为1.5 mg/L 6-BA和0.5 mg/L NAA,芽的平均数量较多,为11.898个;最佳的芽增殖培养基为2 mg/L 6-BA和0.5 mg/L NAA;添加0.5 mg/L IBA有利于芽苗生根,生根率较高,根粗壮,且根系较发达。将幼苗根植于V_(泥炭土)∶V_(蛭石)∶V_(珍珠岩)=3∶1∶0.5的混合基质,成活率达到97.27%。In this study,the underground bulbs of Lilium lancifolium from Xizang were used as experimental materials,and the scales of different inoculation methods,different parts and different growth years were used to explore the in vitro rapid propagation system with high reproduction coefficient.The results showed that the bud induction effect of inner scale of Lilium lancifolium was better and the contamination rate was lower.The optimal inoculation method was adaxial plane down,which could significantly increase the number of bud induction.The longitudinal striating treatment was beneficial to the bud induction of scales.The effect of scale induction in three years of growth was better,and the induced adventitious buds were more robust and grew well.The optimal scale induction medium was 1.5 mg/L 6-BA and 0.5 mg/L NAA,and the average number of buds was 11.898.The optimal bud growth medium was 2 mg/L 6-BA and 0.5 mg/L NAA.The addition of 0.5 mg/L IBA was conducive to the rooting of the sprouts,the rooting rate was higher,the roots were strong and the roots were more developed.The survival rate of seedlings was 97.27%when they were planted in a mixture of V_(peat)soil to V_(vermiculite)to V_(perlite)=3∶1∶0.5.

关 键 词：卷丹百合 鳞片 组织培养 再生体系 外植体 

分 类 号：S682.203.6[农业科学—观赏园艺]