间歇性投加Fe(Ⅱ)协同MBR强化生物脱氮除磷的研究  

Effect of Intermittent Fe(ll)-dosed on the Enhancement of Biological Nitrogen and Phosphorus Removal

作　　者：任宝玉 钱超鸿 REN Baoyu;Qian Chaohong(China Construction Third Bureau Green Industry Investment Co.,Ltd,Wuhan 430064,China)

机构地区：[1]中建三局绿色产业投资有限公司,湖北武汉430064

出　　处：《水处理技术》2025年第1期108-113,共6页Technology of Water Treatment

基　　金：深圳市科学创新委员会基础研究项目(JCYJ20190813163401660)。

摘　　要：为考察间歇性投加亚铁(Fe(Ⅱ))对陶瓷膜膜生物反应器(MBR)脱氮除磷效果的影响,以合成废水作为原水,系统考察了当Fe(Ⅱ)的投加量为Fe(Ⅱ)/P=1和2(摩尔比)时,MBR的出水水质、微生物群落结构及相关功能基因拷贝数的变化情况。结果显示,投加Fe(Ⅱ)可有效去除出水总磷(TP)和总氮(TN),与对照组相比,当投加量为Fe(Ⅱ)/P=1和2时,TP的去除率分别提高了14.40%和18.60%,TN的去除率分别提高了2.30%和6.50%。实验进一步研究了Fe(Ⅱ)对优势菌群和相关功能基因的影响,投加Fe(Ⅱ)可以通过提高脱氮(Dechloromonas、Hyphomicrobium、Nitrospira、Zoogloea和Acinetobacter)和除磷(Candidatus Accumulibacter)微生物的相对丰度、脱氮相关功能基因拷贝数(AOB-amoA、narG、nirK/nirS、norB和norZ)强化生物脱氮除磷效能。采用PICRUSt功能预测分析表明,在Fe(Ⅱ)/P=2条件下,Fe(Ⅱ)可以通过促进反硝化过程的酶(NR、NIR、NOR和N2OR)的相对丰度提高脱氮性能。综上,当投加量为Fe(Ⅱ)/P=2时,可通过影响脱氮除磷过程中关键功能基因拷贝数及生物群落结构,强化陶瓷膜MBR系统生物脱氮除磷性能。This study aimed to clarify the effect of intermittent Fe(II)addition on nitrogen and phosphorus removal in a ceramic membrane bioreactor(MBR)using synthetic wastewater as the influent.The nutrient removal efficiency,microbial community structure,and the copy number of related functional genes were systematically investigated at Fe/P molar ratios of 1 and 2.The results showed that with Fe(II)addition(Fe(II)/P=1 and 2),total phosphorus(TP)removal increased by 14.40%and 18.60%,respectively,while total nitrogen(TN)removal improved by 2.30%and 6.50%,respectively.The study further examined the effects of Fe(II)addition on the dominant bacterial species and the biological nitrogen and phosphorus removal process in activated sludge.The addition of Fe(II)enhanced the removal efficiency by increasing the relative abundance of key microbial strains(Dechloromonas,Hyphomicrobium,Nitrospira,Zoogloea,and Acinetobacter)and boosting the copy number of nitrogen removal-related functional genes(AOB-amoA,narG,nirK/nirS,norB,and norZ).Functional predictions using PICRUSt analysis revealed that Fe(II)/P=2 treatment promoted the relative abundance of nitrogen removal-related enzymes(NR,NIR,NOR,and N2OR),thereby improving nitrogen removal performance.In conclusion,Fe(II)addition at a molar ratio of 2 significantly enhanced biological nitrogen and phosphorus removal by influencing the copy number of key functional genes and altering the microbial community structure.

关 键 词：MBR 亚铁 生物脱氮除磷性能 群落结构 脱氮功能基因 

分 类 号：X703[环境科学与工程—环境工程]