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作 者:李欣 张芝元[1] 韩燕峰[1] LI Xin;ZHANG Zhi-yuan;HAN Yan-feng(Inst.of Fungi Res.,Coll.of Life Sci.,Guizhou Uni.,Guiyang 550025)
机构地区:[1]贵州大学生命科学学院真菌资源研究所,贵州贵阳550025
出 处:《微生物学杂志》2024年第6期15-21,共7页Journal of Microbiology
基 金:国家自然科学基金项目(32060011,32260003);贵州省高层次创新型人才培养计划项目(黔科合平台人才[2020]6005);贵州省生物学一流学科建设项目(GNYL[2017]009)。
摘 要:调查我国拟单胞瓶霉属(Phialemoniopsis)物种资源。采用经典形态学分类及分子系统学方法对分离获得的菌株进行鉴定。从海南省海口市海南大学海淀校区和江西省吉安市骡子山生态公园的绿化带土壤中采集获得6株拟单胞瓶霉属菌株。菌株GZUIFR 21.872和GZUIFR 21.873与角膜拟单胞瓶霉(Phialemoniopsis ocularis)的形态特征非常相近,且系统发育上与该种的模式菌株以较高的支持率聚在一个亚分支;菌株GZUIFR 21.874和GZUIFR 21.875与内生拟单胞瓶霉(Phialemoniopsis endophytica)的形态特征非常相近,且系统发育上与该种的模式菌株以较高的支持率聚在一个亚分支;此外,菌株GZUIFR 21.876和GZUIFR 21.877与多腔拟单胞瓶霉(Phialemoniopsis pluriloculosa)的形态特征非常相近,且系统发育上与该种的模式菌株以较高的支持率聚在一个亚分支。调查结果表明,角膜拟单胞瓶霉和多腔拟单胞瓶霉为中国新记录种;内生拟单胞瓶霉首次分离自土壤,营腐生生活,这三个拟单胞瓶霉属成员的发现进一步丰富了我国拟单胞瓶霉属的物种资源。The species resources of Phialemoniopsis in China were investigated.Classic morphology characteristic and molecular phylogenetic analyses were used to identify the isolates.Six strains of the genus Phialemoniopsis were obtained from soil of the green belt of Hainan University Haidian Campus in Haikou,Hainan Province and Mt.Mule Ecological Park in Ji′an,Jiangxi Province.Strains GZUIFR 21.872 and GZUIFR 21.873 were very similar to the original description of Phialemoniopsis ocularis in morphology and clustered into a subclade with the sequences of type strain of P.ocularis in phylogenetic tree.Strains GZUIFR 21.874 and GZUIFR 21.875 were very similar to the original description of Phialemoniopsis endophytica in morphology and clustered into a subclade with the sequences of type strain of P.endophytica in phylogenetic tree.Strains GZUIFR 21.876 and GZUIFR 21.877 were very similar to the original description of Phialemoniopsis pluriloculosa in morphology and clustered into a subclade with the sequences of type strain of P.pluriloculosa in phylogenetic tree.P.ocularis and P.pluriloculosa are new record strains to China,P.endophytica was first isolated from the soil and is saprophytic,the discovery of these three members further enriched the species resources of the genus in our country.
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