碳源对球孢白僵菌分生孢子质量的影响  

Effects of Carbon Sources on Conidial Quality of Beauveria bassiana

作　　者：陈金峰 龙静 樊可 张静姝 CHEN Jin-feng;LONG Jing;FAN Ke;ZHANG Jing-shu(Coll.of Biol.&Chem.Engin.,2.Coll.of Mod.Health Indust.,Chongqing 2nd Uni.of Educ.,Chongqing 400067)

机构地区：[1]重庆第二师范学院生物与化学工程学院,重庆400067 [2]重庆第二师范学院大健康产业学院,重庆400067

出　　处：《微生物学杂志》2024年第6期62-68,共7页Journal of Microbiology

基　　金：重庆市自然科学基金项目(CSTB2022NSCQ-MSX0261);重庆市教育委员会科学技术计划重点研究项目(KJZD-K202201601)。

摘　　要：分生孢子对球孢白僵菌(Beauveria bassiana)的繁殖和毒力至关重要。为提高分生孢子质量,将球孢白僵菌接种于不同碳源PDA培养基上,分析不同碳源对分生孢子细胞壁结构成分、细胞内抗氧化酶活性、海藻糖含量和相关基因表达的影响,并进一步探究碳源和调控分生孢子发育基因的关系。结果显示,以葡萄糖为对照,果糖、麦芽糖、蔗糖为碳源培养的分生孢子细胞壁多糖、细胞内抗氧化酶和海藻糖合成酶以及分生孢子发育等相关基因的表达量被显著上调;多糖含量和抗氧化酶活性为果糖>麦芽糖>蔗糖>葡萄糖>乳糖,海藻糖含量为蔗糖>果糖>麦芽糖>葡萄糖>乳糖。通过探究碳源对分生孢子质量的影响,为真菌的抗逆筛选提供参考。The quality of conidia imperatively affects proliferate and the virulence of Beauveria bassiana.In order to raise the conidial quality of B.bassiana,the fungi were cultured on PDA medium with different carbon sources.The effects of carbon sources on conidial quality were clarified based on conidial wall components,intracellular antioxidant enzyme activities,endogenous trehalose content,and relative genes expressions.The relationship between the carbon sources and the gene associated with conidial development was further analyzed.The results showed that,glucose was used as a control,the expression levels of genes related to conidial wall composition,antioxidant enzymes,trehalose synthase and conidial development in conidia cultured with fructose,maltose,and sucrose as carbon sources were significantly up-regulated.Polysaccharide content and antioxidant enzyme activity were successively as followed fructose>maltose>sucrose>glucose>lactose,and the trehalose content was successively as followed sucrose>fructose>maltose>glucose>lactose.By exploring the effect of carbon source on the quality of conidia,it provided a theoretical basis for the screening highly resistant conidia.

关 键 词：球孢白僵菌 碳源 分生孢子 细胞壁 质量 

分 类 号：Q939.1[生物学—微生物学]