积雪草酸对去卵巢骨质疏松大鼠骨质疏松的改善作用  

Role of asiatic acid in treating osteoporosis in ovariectomized osteoporotic rats

作　　者：罗燕宁 洪建仁 余双奇 LUO Yanning;HONG Jianren;YU Shuangqi(Department of Hand and Foot Microsurgery,Huangshi Central Hospital,the Affiliated Hospital of Hubei Polytechnic University,Huangshi,Hubei 435000,China;Department of Spinal Surgery,Wuhan Puren Hospital,Wuhan,Hubei 430081,China)

机构地区：[1]黄石市中心医院,湖北理工学院附属医院手足显微外科,湖北黄石435000 [2]武汉市普仁医院脊柱外科,湖北武汉430081

出　　处：《中华实用诊断与治疗杂志》2024年第12期1210-1215,共6页Journal of Chinese Practical Diagnosis and Therapy

基　　金：湖北省卫生健康委科研立项项目(WJ2021F015)。

摘　　要：目的探讨积雪草酸对骨保护素(OPG)/核因子-κB受体活化因子配体(RANKL)信号通路的影响及其对去卵巢骨质疏松大鼠骨质疏松的改善作用。方法雌性SD大鼠40只,体质量200~220 g,随机分为假手术组、模型组、积雪草酸组、雌二醇组各10只。模型组、积雪草酸组、雌二醇组大鼠采用卵巢切除法制备骨质疏松模型,假手术组仅打开腹腔,不切除卵巢;造模成功后积雪草酸组大鼠灌胃积雪草酸混悬液120 mg/kg,雌二醇组大鼠灌胃雌二醇片混悬液0.184 mg/kg,假手术组和模型组大鼠灌胃等量生理盐水,均1次/d,连续12周。测量各组大鼠左侧股骨近端骨密度(BMD),采用ELISA法检测血清雌二醇、骨形成标志物[骨钙素(BGP)、Ⅰ型前胶原氨基端原肽(PⅠNP)]、骨吸收标志物[Ⅰ型胶原C端肽(CTX-Ⅰ)、抗酒石酸酸性磷酸酶5b(TRAP-5b)]水平,行micro-CT检查测量右侧股骨骨体积分数(BV/TV)、骨小梁数量(Tb.N)、骨小梁厚度(Tb.Th),采用三点弯曲法测量右侧股骨生物力学,采用Western blot法检测右侧股骨组织OPG、RANKL、核因子-κB受体活化因子(RANK)蛋白相对表达量。结果药物干预前、后,4组左侧股骨BMD及血清雌二醇水平比较差异均有统计学意义(F=31.570~63.726,P均<0.05);药物干预后,假手术组、雌二醇组、积雪草酸组、模型组左侧股骨BMD[(241.95±27.35)、(210.77±19.63)、(173.49±18.54)、(108.32±24.43)g/cm2]及血清雌二醇水平[(475.93±84.50)、(395.67±52.83)、(321.88±46.42)、(215.37±31.05)ng/L]均依次降低(P<0.05),积雪草酸组、雌二醇组均高于药物干预前(P<0.05),假手术组、模型组与药物干预前比较差异均无统计学意义(P>0.05)。4组血清BGP、PⅠNP、TRAP-5b、CTX-Ⅰ水平,右侧股骨BV/TV、Tb.N、Tb.Th、最大载荷、断裂挠度及RANKL、RANK、OPG蛋白相对表达量比较差异均有统计学意义(F=14.556~332.330,P均<0.05);假手术组血清BGP[(4.95±0.34)ng/L]、PⅠNP[(33.99±4.21)μg/L]水平�Objective To investigate the influence of asiatic acid on osteoprotegerin(OPG)/receptor activator of nuclear factor-κB ligand(RANKL)signaling pathway and its role in treating osteoporosis in ovariectomized osteoporotic rats.Methods Forty female SD rats,weighing 200-220g,were randomly divided into the sham-surgery group,model group,asiatic acid group and estradiol group,with 10rats in each group.The osteoporosis models were established in the model group,asiatic acid group and estradiol group by ovariectomy.The rats in the sham-surgery group only had the abdominal cavities opened without removing the ovaries.After successful modeling,the rats in the asiatic acid group received gavage with 120mg/kg asiatic acid suspension,the rats in the estradiol group received gavage with 0.184mg/kg estradiol tablet suspension,and the rats in the sham-surgery group and model group received gavage with the same amount of normal saline,once a day for 12weeks.The bone mineral density(BMD)of the left proximal femur was measuredD?in each group.ELISA was used to detect the serum levels of estradiol,bone formation markers[bone gamma-carboxyglutamic acid protein(BGP),procollagen typeⅠN-terminal propeptide(PⅠNP)],and bone resorption markers[C-telopeptide of typeⅠcollagen(CTX-Ⅰ),tartrate-resistant acid phosphatase-5b(TRAP-5b)].Micro-CT scan was done to measure the right femur bone volume fraction(BV/TV),trabecular number(Tb.N)and trabecular thickness(Tb.Th);three-point bending method was used to measure the biomechanics;Western blot was used to detect the relative expressions of OPG,RANKL and receptor activator of nuclear factor-κB(RANK)proteins.Results Before and after drug intervention,the left femur BMDs and serum estradiol levels showed significant differences among four groups(F=31.570-63.726,all P values<0.05).After drug intervention,the BMDs[(241.95±27.35),(210.77±19.63),(173.49±18.54),(108.32±24.43)g/cm2]and serum estradiol levels[(475.93±84.50),(395.67±52.83),(321.88±46.42),(215.37±31.05)ng/L]decreased sequential

关 键 词：骨质疏松 积雪草酸 骨保护素/核因子-κB受体活化因子配体信号通路 去卵巢 大鼠 

分 类 号：R285.5[医药卫生—中药学]