糯谷猪PRKAA1基因表达与肉用性状关联研究  

Correlation between PRKAA1 gene expression level and meat traits in Nuogu pigs

作　　者：张雄 史开志 杨齐心 周思旋 吴玙彤 李冬光 赵春萍 ZHANG Xiong;SHI Kaizhi;YANG Qixin;ZHOU Sixuan;WU Yutong;LI Dongguang;ZHAO Chunping(Institute of Guizhou Animal Husbandry and Veterinary Science,Guiyang 550005,China;Guizhou Province Livestock and Poultry Genetic Resources Management Station,Guiyang 550004,China)

机构地区：[1]贵州省农业科学院畜牧兽医研究所,贵州贵阳550005 [2]贵州省畜禽遗传资源管理站,贵州贵阳550004

出　　处：《畜牧与兽医》2025年第1期7-10,共4页Animal Husbandry & Veterinary Medicine

基　　金：贵州省科技计划项目(黔科合支撑[2020]1Y031号,黔科合支撑[2022]032号,黔科合支撑[2023]一般154号);贵州省生猪产业技术体系建设专项(GZSZCYJSTX);贵州省农业计划项目(黔财农[2023]82号);贵州省农业科学院青年基金项目(黔农科院青年基金[2023]21号)。

摘　　要：旨在掌握贵州糯谷猪胴体和肉质性状表型差异,分析单磷酸腺苷活化蛋白激酶α1亚基(PRKAA1)基因mRNA表达量和肉用性状关联性。以糯谷猪为研究对象,分别对10月龄的9头去势公猪和10头母猪进行屠宰测定,采用实时荧光定量PCR检测糯谷猪背最长肌组织PRKAA1基因mRNA表达量,并与测定性状进行相关性分析。结果:去势公猪和母猪宰前活重分别为(94.78±8.75)kg和(93.10±9.23)kg,两者差异不显著(P>0.05),但母猪皮率和瘦肉率指标分别高于去势公猪21.91%和8.74%,且差异显著(P<0.05),肥肉率指标极显著低于去势公猪(P<0.01);关联分析发现,糯谷猪母猪背最长肌组织PRKAA1基因mRNA表达水平与肩部最厚处背膘厚呈极显著负相关性(P<0.01)。本研究分析了性别对糯谷猪肉用性状的影响,发现PRKAA1可作为调控糯谷猪胴体性状的候选基因。This study was to understand the phenotypic differences in carcass and meat quality traits of Guizhou Nuogu pigs,and to analyze the correlation between PRKAA1 gene mRNA expression and meat quality traits.Nuogu pigs were selected as the research objects.The car-cass and meat quality indexes of 9 castrated boars and 10 sows of Nuogu pigs were determined,and the mRNA expression level of the longis-simus dorsi muscle tissue of the pigs was detected by real-time quantitative PCR(RT-qPCR),and the correlation between their PRKAA1 gene mRNA expression level and meat quality traits was analyzed.The results showed that there were no significant differences in pre-slaugh-ter live weight between the castrated boars(9478±875)kg and sows(9310±923)kg.The indexes of skin percentage and lean meat percentage of the sows were significantly higher than those of the castrated boars by 2191%and 874%(P<005),respectively;but their fat meat percentage was extreme significantly lower than that of the castrated boars(P<001).The correlation analysis showed that there was a significant negative correlation between the PRKAA1 gene mRNA expression level of the longissimus dorsalis muscle at the back and back-fat thickness at the thickest part of the shoulder of the sows(P<001).In summary,this study analyzed the effect of gender on the meat per-formance of Nuogu pigs and confirmed that PRKAA1 could be used as a candidate gene for regulating the carcass traits of Nuogu pigs.

关 键 词：糯谷猪 PRKAA1基因 胴体 肉质 

分 类 号：S828[农业科学—畜牧学]