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作 者:王凯月 姜丹丹 郑宇航 李均同[2] 吴香菊 胡悦 齐静[2] 丛晓燕[2] 孙英峰 杜以军[2] WANG Kaiyue;JIANG Dandan;ZHENG Yuhang;LI Juntong;WU Xiangju;HU Yue;QI Jing;CONG Xiaoyan;SUN Yingfeng;DU Yijun(Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry,College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300392,China;Shandong Key Laboratory of Animal Disease Control and Breeding,Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Sciences,Jinan 250100,China;College of Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,China)
机构地区:[1]天津农学院动物科学与动物医学学院/天津市农业动物繁育与健康养殖重点实验室,天津300392 [2]山东省农业科学院畜牧兽医研究所/山东省畜禽疫病防治与繁育重点实验室,山东济南250100 [3]青岛农业大学动物医学院,山东青岛266109
出 处:《畜牧与兽医》2025年第1期79-85,共7页Animal Husbandry & Veterinary Medicine
基 金:山东省自然科学基金项目(ZR2021MC139,ZR2023MC076);国家自然科学基金项目(32102710);国家兽用生物制品工程技术研究中心开放课题项目(GTKF(23)009)。
摘 要:猪繁殖与呼吸综合征(PRRS)是由猪繁殖与呼吸综合征病毒(PRRSV)感染引起的一种急性、高度接触性传染病,5-氨基乙酰丙酸(5-ALA)是一种天然氨基酸,具有重要的抗病毒功能,能够抑制多种病毒的复制。为了研究5-ALA抗PRRSV作用,通过CCK-8法检测5-ALA对永生化猪肺泡巨噬细胞(PAM-KNU)的细胞毒性,通过预防、直接杀灭和治疗3种给药方式来判定5-ALA抑制PRRSV增殖的最佳作用方式和最佳作用浓度。结果:当药物浓度为1 mmol/L时,病毒滴度及其cDNA拷贝数水平显著下调,同时,5-ALA对PRRSV的预防作用和直接杀灭作用优于其治疗作用;从吸附、内化、早期胞内复制3个环节检测5-ALA对PRRSV复制周期的影响,结果表明5-ALA在感染细胞的内化环节和早期胞内复制环节起作用。本研究证实了5-ALA可以抑制PRRSV复制,为预防和治疗PRRSV感染提供了理论基础。Porcine reproductive and respiratory syndrome(PRRS)is an acute,highly contagious disease caused by porcine reproductive and respiratory syndrome virus(PRRSV).5-aminolevulinic acid(5-ALA),as a natural amino acid,plays important antiviral function,and it inhibits replication of multiple viruses.However,whether 5-ALA can exert anti-PRRSV effect has not been reported,to our knowledge.In this study,the cytotoxicity of 5-ALA on PAM-KNU cells was detected by CCK-8 assay.The optimal method for and the optimal concentra⁃tion of 5-ALA to inhibit PRRSV propagation were determined by three modes of administration:prevention,direct killing and treatment.The results showed that the viral titers and cDNA copies were significantly down-regulated when the 5-ALA concentration was 1 mmol/L.Mean⁃while,the anti-PRRSV effect of prevention and direct killing of 5-ALA were more effective than the function of treatment.The effect of 5-ALA on PRRSV replication cycle was investigated by three periods:adsorption,internalization and early intracellular replication.The results showed that 5-ALA played a key role in the internalization and early intracellular replication period to inhibit PRRSV replication.Our study suggested that 5-ALA could inhibit PRRSV replication,which might serve as theoretical support for the prevention and treatment of PRRSV infection.
关 键 词:猪繁殖与呼吸综合征病毒 5-氨基乙酰丙酸 抗病毒
分 类 号:S858[农业科学—临床兽医学]
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