根皮素包合物对ARPE-19细胞损伤的保护效果的研究  

Study on the Protective Effect of Phloretin Inclusion Complexes on ARPE-19 Cell Damage

作　　者：郭志想 杨光[1] 杨波[1] 王凯[1] GUO Zhixiang;YANG Guang;YANG Bo;WANG Kai(College of Health Sciences and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海理工大学健康科学与工程学院,上海200093

出　　处：《工业微生物》2024年第6期75-81,共7页Industrial Microbiology

摘　　要：为探究根皮素(Phloretin,Ph)及根皮素/羟丙基-β-环糊精包合物(Ph/HBC-IC)对甲基乙二醛(MGO)诱导的人视网膜色素上皮细胞(ARPE-19)损伤的保护效果,文章采用冷冻干燥法制备得到Ph/HBC-IC,并利用傅里叶红外光谱(FTIR)、扫描电镜(SEM)、X射线衍射(XRD)等技术对包合物进行表征;通过CCK-8法检测细胞活力,筛选得到MGO诱导ARPE-19细胞损伤的最佳质量分数、Ph及Ph/HBC-IC的最佳干预质量分数,并考察了Ph及Ph/HBC-IC对MGO诱导ARPE-19细胞损伤的保护效果。研究结果显示,上述三种表征方式均能直接或间接证明包合物的形成;建立细胞损伤模型的最佳浓度是1 mmol/L MGO,且Ph及Ph/HBC-IC在0~15 mg/L的质量浓度范围内对细胞存活率没有显著影响。进一步研究表明,诱导的ARPE-19细胞经15 mg/L Ph及Ph/HBC-IC预处理后,细胞存活率分别提高了20.4%和28.67%,证实了Ph及Ph/HBC-IC对MGO诱导的细胞损伤具有保护作用,且Ph/HBC-IC对细胞的保护效果更好。研究结果为Ph及Ph/HBC-IC在相关领域的开发应用提供了科学依据。To investigate the protective effect of Phloretin(Ph)and Phloretin/hydroxypropyl-β-cyclodextrin inclusion complex(Ph/HBC-IC)on methylglyoxal(MGO)-induced damage to human retinal pigment epithelial cells(ARPE-19),the paper prepared Ph/HBC-IC by freeze-drying method and characterized the inclusion complex using Fourier transform infrared spectroscopy(FTIR),scanning electron microscopy(SEM),X-ray diffraction(XRD)and other techniques.By using the CCK-8 method to detect cell viability,the paper screened the optimal concentration of MGO induced ARPE-19 cell damage,the optimal intervention concentrations of Ph and Ph/HBC-IC,and investigated the protective effects of Ph and Ph/HBC-IC on MGO induced ARPE-19 cell damage.The research results showed that all three characterization methods mentioned above can intuitively or indirectly prove the formation of inclusion complexes;The optimal concentration for establishing a cell damage model was 1 mmol/LMGO,and pH and pH/HBC-IC had no significant effect on cell survival within the concentration range of 0-15 mg/L.Further research has shown that after pretreatment with 15 mg/L Ph and Ph/HBC-IC,the survival rate of induced ARPE-19 cells increased by 20.4%and 28.67%,respectively,confirming that Ph and Ph/HBC-IC had a protective effect on MGO induced cell damage,and Ph/HBC-IC had a better protective effect on cells.The results provided a scientific basis for the development and applications of Ph and Ph/HBC-IC in related fields.

关 键 词：根皮素 包合物 甲基乙二醛 人视网膜上皮细胞(ARPE-19) 保护效果 

分 类 号：R285[医药卫生—中药学]