肠道菌群失调对小鼠脾脏和胸腺中Th17细胞数量的影响  

Effect of Intestinal Dysbacteriosis on the Number of Th17 Cells in the Spleen and Thymus of Mice

作　　者：李成喜 孙怡慧 韩金雪 张娜娜 LI Chengxi;Sun Yihui;HAN Jinxue;ZHANG Nana(Anhui Key Laboratory of Infection and Immunity,Department of Microbiology,Bengbu Medical University,Bengbu,Anhui,233000 China)

机构地区：[1]蚌埠医科大学感染与免疫安徽省重点实验室/微生物教研室,安徽蚌埠233000

出　　处：《工业微生物》2024年第6期115-117,共3页Industrial Microbiology

基　　金：校级重点项目“肠道菌群损耗对小鼠小肠中Th17细胞分化的影响机制”(编号:2020byzd032)。

摘　　要：Th17细胞通常以微生物依赖的模式存在于肠道中,对维持肠道屏障的完整性和稳定性具有重要作用。当肠道菌群异常时,Th17细胞的数量会发生变化,进而引起肠道疾病和免疫失衡。文章通过使用混合抗生素饲养小鼠来构建菌群失调模型,并采用流式细胞仪分别检测实验组和对照组小鼠胸腺和脾脏中Th17细胞数量的占比。实验结果显示,混合抗生素喂养前期,实验组小鼠的体重明显下降,后期逐渐恢复。流式检测结果显示,实验组小鼠胸腺和脾脏的Th17细胞占比与对照组相比发生了显著下降,下降比率分别达到了47.70%和23.83%。研究结果表明,由混合抗生素饲养引起的小鼠肠道菌群失调,会导致小鼠胸腺和脾脏中Th17细胞数量的降低。Th17 is typically present in the gut in a microbe-dependent manner and plays a crucial role in maintaining the integrity and stability of intestinal barrier.When the intestinal flora is abnormal,the number of Th17 cells will change,leading to intestinal diseases and immune imbalances.The artide employed a microbial dysbiosis model in mice by administering a combination of antibiotics and examined the percentage of Th17 cells in the thymus and spleen of mice in experimental and control groups by flow cytometry.It was observed that the mice in the experimental group exhibited a notable decline in weight during the initial phase of mixed antibiotic feeding,which gradually recovered in the subsequent stage.The results of flow cytometry demonstrated a notable decline in the percentage of Th17 cells in the thymus and spleen of the experimental group of mice,exhibiting a reduction of 47.70%and 23.83%,respectively,in comparison to the control group.These findings indicated that the intestinal dysbacteriosis of mice resulting from mixed antibiotic feeding can contribute to a reduction in the number of Th17 cells in the thymus and spleen of mice.

关 键 词：肠道菌群 Th17细胞 菌群失调 胸腺 脾脏 

分 类 号：R392[医药卫生—免疫学]