甲醛隔离液在心血管病理标本中的应用效果  

Application effect of formaldehyde isolation solution in cardiovascular pathological specimens

作　　者：张梦思 刘建英 周桥 ZHANG Mengsi;LIU Jianying;ZHOU Qiao(Department of Pathology,Wuhan Asia General Hospital,Wuhan 430000;Pathological Medicine Teaching and Research Center,Wuhan University of Science and Technology Affiliated Hospital,Wuhan 430000,China)

机构地区：[1]武汉亚心总医院病理科,武汉430000 [2]武汉科技大学附属医院病理医学教学与研究中心,武汉430000

出　　处：《临床与病理杂志》2024年第8期1115-1120,共6页Journal of Clinical and Pathological Research

摘　　要：目的:甲醛作为病理标本固定液性能良好,但它是一种具有强烈刺激性的挥发性液体,不仅污染环境,而且危害人体健康。本研究尝试在甲醛中加入隔离液以降低其挥发,并评估甲醛隔离液在心血管病理标本中的应用效果。方法:收集2024年1月1日至4月30日武汉亚洲心脏病医院(循礼门院区)和武汉亚心总医院(沌口院区)外科手术切除的新鲜心血管病理标本各20例。采用4%中性甲醛固定,其中武汉亚心总医院(沌口院区)在标本瓶中加入一层异构烷烃作为液态隔离层。在病理检查结束后,由3名病理医师对2家医院的同种标本的苏木精-伊红(hematoxylineosin,HE)染色进行评分。结果:2家医院同种标本的HE染色评分差异没有统计学意义(P>0.05)。加入了隔离液的甲醛固定液上方空气的甲醛浓度低于未加隔离液的甲醛固定液。结论:甲醛隔离液对心血管病理标本的HE染色没有明显影响,且可以阻止甲醛的挥发,减少甲醛对医生的伤害。Objective:Formaldehyde is an effective fixative for pathological specimens,but it is a highly volatile and irritating liquid that poses environment and health hazards.This study explores the addition of an isolation solution to formaldehyde to reduce its volatility and evaluates the application effect of formaldehyde isolation solution in cardiovascular pathological specimens.Methods:A total of 20 fresh cardiovascular pathological specimens from surgeries at Wuhan Asia Heart Hospital(Xunlimen Campus)and Wuhan Asia General Hospital(Zhuankou Campus)from January 1 to April 30,2024,were collected.Specimens were fixed with 4%neutral formaldehyde,with the addition of a layer of isoparaffins as a liquid isolation layer in the specimen bottles at the Zhuankou Campus.After pathological examination,3 pathologists scored the hematoxylin-eosin(HE)staining of the same type of specimens from both hospitals.Results:There was no statistically significant difference in the HE staining scores of the same type of specimens between the 2 hospitals(P>0.05).Formaldehyde with the added isolation solution showed lower formaldehyde concentration in the air above the solution compared to formaldehyde without the isolation layer.Conclusion:The formaldehyde isolation solution does not significantly affect HE staining of cardiovascular pathological specimens and effectively reduces formaldehyde volatilization,minimizing harm to medical staff.

关 键 词：甲醛 异构烷烃 液态隔离层 苏木精-伊红染色 

分 类 号：R54[医药卫生—心血管疾病]