牡丹根际多功能促生菌的筛选及其促生潜力分析  

Screening of Multifunctional Growth-promoting Bacteria in Tree Peony Rhizosphere and Analysis of Their Promoting Potential

作　　者：卢明媚 刘萍 石犇 杨瑞先 LU Mingmei;LIU Ping;SHI Ben;YANG Ruixian(Department of Environmental Engineering and Chemistry,Luoyang Institute of Science and Technology,Luoyang,Henan 471023)

机构地区：[1]洛阳理工学院环境工程与化学学院,河南洛阳471023

出　　处：《北方园艺》2024年第24期45-53,共9页Northern Horticulture

基　　金：洛阳理工学院高层次人才启动资助项目(2019BZ14)。

摘　　要：以牡丹根际土壤为试材,采用筛选培养基对牡丹根际促生菌进行分离纯化,对筛选所得促生菌株的固氮、溶磷、分泌吲哚乙酸(IAA)、产铁载体、1-氨基环丙烷-1-羧酸(ACC)脱氨酶活性等促生特性进行检测,同时利用盆栽试验分析筛选菌株对小白菜幼苗的促生效果,以期从牡丹根际筛选出具有多种促生功能的菌株,为开发高效微生物菌剂提供参考依据和优良菌种资源。结果表明:从牡丹根际土壤中筛选出8株多功能促生菌株,其中菌株N3、P2和N16兼具固氮、溶磷、产铁载体、分泌IAA能力,且菌株N3分泌IAA含量最高,为27.90 mg·L^(-1),P2溶解无机磷能力最强,溶磷圈直径/菌落直径(D/d)为3.77,其他菌株(L1、L5、L10、K5和N26)均具有2种及以上促生特性。菌株16S rDNA序列测定及系统发育分析结果表明菌株N3和P2属于泛菌属(Pantoea),菌株L1、L5和K5属于假单胞菌属(Pseudomonas),N16属于假黄色单胞菌属(Pseudoxanthomonas),L10和N26属于农杆菌属(Agrobacterium)。盆栽试验结果显示4株代表菌株对小白菜均有一定的促生效果,表明牡丹根际促生菌具有制备微生物肥料的潜力。The rhizosphere soil of tree peony was used as the experimental material,the tree peony rhizosphere growth promoting bacteria were isolated and purified by the screening medium.The growth promoting characteristics of the screened strains,such as nitrogen fixation,phosphorus solubilization,indole-3-acetic acid(IAA)secretion,iron carrier production,and 1-aminocyclopropane-1-carboxylic acid(ACC)deaminase activity,were studied.At the same time,pot cultivation experiments were used to analyze the growth promoting effect of the screened strains on Chinese cabbage seedlings,in order to screen strains with multiple growth promoting functions from the tree peony rhizosphere and provide reference and excellent bacterial resources for the development of efficient microbial agents.The results showed that eight composite growth promoting strains were screened from the tree peony rhizosphere soil.Among them,N3,P2,and N16 showed the ability of nitrogen-fixing,phosphate-dissolving,siderophore and IAA production.The highest content of IAA secreted by N3 was 27.90 mg·L^(-1).P2 showed the strongest ability to dissolve inorganic phosphorus,and the ratio of the phosphorus solubilizing circle diameter and colony diameter(D/d)was 3.77.Other strains(L1,L5,L10,K5,and N26)all showed two or more growth promoting characteristics.The 16S rDNA sequencing and phylogenetic analysis of these strains indicated that N3 and P2 belonged to the genus Pantoea,Ll,L5 and K5 belonged to the genus Pseudomonas,N16 belonged to the genus Pseudoranthomonas,L1o and N26 belonged to the genus Agrobacterium.The results of the pot experiment showed that four representative strains had a certain promoting effect on the growth of Chinese cabbage,indicating that tree peony rhizosphere promoting bacteria had the potential to prepare microbial fertilizers.

关 键 词：牡丹 根际促生菌 促生特性 微生物肥料 

分 类 号：S685.11[农业科学—观赏园艺]