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作 者:刘清熔 侯银梦 陈卓[1,2] 陈晓虹 LIU Qing-Rong;HOU Yin-Meng;CHEN Zhuo;CHEN Xiao-Hong(College of Life Science,Henan Normal University,Xinxiang 453007;The Observation and Research Field Station of Taihang Mountain Forest Ecosystems of Henan Province,Xinxiang 453007,China)
机构地区:[1]河南师范大学生命科学学院,新乡453007 [2]河南省太行山森林生态系统野外科学观测研究站,新乡453007
出 处:《动物学杂志》2024年第6期974-980,共7页Chinese Journal of Zoology
基 金:中央财政国家重点野生动植物保护资助项目。
摘 要:2023年7月5日,在河南省信阳市商城县黄柏山国家森林公园捡拾到1号死亡的蛇类标本。综合形态测量、鳞被比对、线粒体COI基因分子系统关系和生态资料,鉴定为大别山脊蛇(Achalinus dabieshanensis),系河南省蛇类分布新记录种。该新标本颞鳞1/(1+1)+2+2,与模式标本的颞鳞2+2+3存在形态差异,且二者间的遗传距离为0.002。大别山脊蛇在河南的发现扩展了对其地理分布的认知,丰富了河南大别山区蛇类多样性,也为该物种的谱系地理学研究提供了基础资料。The Achalinus dabieshanensis was initially described as a new species in 2023.It is only known to be located in Anhui Province,China.This study provides morphological and molecular information of A.dabieshanensis,which was collected in Huangbaishan National Forest Park,Henan Province,China.In this study,22 morphological indexes were selected for morphological comparison.The length was measured by vernier caliper,and the number of scales was described manually.Genomic DNA was extracted from liver tissue(preserved in 95%alcohol)using FastPure Gel DNA Extraction Mini Kit,cytochrome c oxidase subunit I(COI)was targeted and amplified using primers Chmf4 and Chmr4.Sequences of 21 recognized species of genus Achalinus and one outgroup(Fimbrio klossi)were downloaded from GenBank(Table 1).Uncorrected pairwise distances(p-distance)among closely related congeners were calculated using MEGA 7.0 software.Phylogenetic tree was constructed by Bayesian inference(BI)analysis using PhyloSuite v1.2.2 software.The body measurements and scale morphology of the collected specimen showed little difference compared with A.dabieshanensis from type locality.The temporal of this specimen is 1/(1+1)+2+2,which is morphologically different from the temporal(2+2+3)of the topotype(Fig.1).Phylogenetic tree indicated that the specimen closely clustered with a topotype of A.dabieshanensis(Lu’an,Anhui,China)(Fig.2).The genetic distance between them is 0.002,significantly smaller than the interspecific genetic distance of Achalinus(0.034﹣0.297)(Appendix 1).The ecological environment of the specimen was similar to that of the topotype(Fig.3).Considering both the morphological characteristics and the molecular phylogenetic relationship,the specimen of Achalinus snake,HNNU2307I021,from Xinyang County,Henan Province was identified as A.dabieshanensis,which was a new provincial record of Henan.
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