水产养殖环境中多种农药残留检测方法研究  

Study on Detection Methods for Multiple Pesticide Residues in Aquaculture Environment

作　　者：张柏瑀[1] 董鸿竺 杜姗姗 张彤 万建春 姜莉 耿庆华 刘瑜 ZHANG Bo-Yu;DONG Hong-Zhu;DU Shan-Shan;ZHANG Tong;WAN Jian-Chun;JIANG Li;GENG Qing-Hua;LIU Yu(Shenyang Heping District Center for Disease Prevention and Control,Shenyang 110005;Technology Center of Shenyang Customs,Shenyang 110016;Technology Center of Nanchang Customs,Nanchang 330000)

机构地区：[1]沈阳市和平区疾病预防控制中心,沈阳110005 [2]沈阳海关技术中心,沈阳110016 [3]南昌海关技术中心,南昌330000

出　　处：《中国口岸科学技术》2024年第12期56-62,共7页China Port Science and Technology

基　　金：海关总署科研计划项目(2022HK021,2024HK113);辽宁省自然科学基金项目(2022HK021)。

摘　　要：本研究采用改进的QuEChERS前处理技术与超高效液相色谱-串联质谱仪,建立了水产养殖环境中32种农药同时测定的分析方法。试样经乙腈提取,改进的QuEChERS净化,采用C_(18)色谱柱分离,流动相为乙腈+甲酸水,质谱扫描模式为多反应监测(MRM),外标法定量。结果显示,该方法的检出限为0.003~0.015 mg/L,定量限为0.01~0.05 mg/L,回收率为75%~113%,相对标准偏差为0.132%~6.73%。结果表明,该方法简便、快速、准确,适用于水产养殖环境中32种农药的同时检测。This study developed an analytical method for the simultaneous determination of 32 pesticides in aquaculture environments using an improved QuEChERS pre-treatment technology coupled with ultra-high performance liquid chromatography-tandem mass spectrometry by optimizing pre-treatment and instrument conditions.The sample was extracted with acetonitrile,purified with improved QuEChERS method,and separated using a C_(18)chromatographic column.The mobile phase consisted of acetonitrile and formic acid water,with mass spectrometry scanning mode operated in multi reaction monitoring(MRM).Quantification was performed using the external standard method.The detection limit of this method ranged from 0.003 to 0.015 mg/L,with a quantification limits from 0.01-0.05 mg/L,recovery rate 75%-113%,relative standard deviation 0.132%-6.73%.This method is simple,fast,and accurate,making it suitable for the simultaneous detection of 32 pesticides in aquaculture environments.

关 键 词：农药 液相色谱-串联质谱 水产养殖环境 改进的QuEChERS 

分 类 号：X839.2[环境科学与工程—环境工程] O657.63[理学—分析化学]