石墨烯量子点荧光探针检测结球甘蓝中的马拉硫磷  

Detection of Malathion in Cabbage by Graphene Quantum Dot Fluorescent Probe

作　　者：莫敏 郑文 明德旺 文方 谢娅芹 张梦婷 李贤良 郗存显 Mo Min;Zheng Wen;Ming De-Wang;Wen Fang;Xie Ya-Qin;Zhang Meng-Ting;Li Xian-Liang;Xi Cun-Xian(Technical Center of Chongqing Customs,Chongqing 401000;Yangtze Normal University,Chongqing 408100)

机构地区：[1]重庆海关技术中心,重庆401000 [2]长江师范学院,重庆408100

出　　处：《中国口岸科学技术》2024年第12期69-76,共8页China Port Science and Technology

基　　金：重庆市技术创新与应用发展专项面上项目(CSTB2022TIAD-GPX0030)。

摘　　要：本研究利用荧光共振能量转移原理(Fluorescence Resonance Energy Transfer,FRET),合成功能化石墨烯荧光探针用于检测结球甘蓝中的马拉硫磷。通过自下而上的方法对丝氨酸(L-Ser)、赖氨酸(L-Lys)和柠檬酸的混合物进行热解反应,得到功能化石墨烯量子点(S-L-GQDs)。其与氨基修饰的马拉硫磷适配体(O-Apt_(m))通过共价连接,生成O-AptmS-L-GQDs连接物,表现出优异的荧光性能。氧化石墨烯(GO)与O-Apt_(m)-S-L-GQDs作用引起荧光淬灭,在目标物马拉硫磷存在下,使得GO从O-Apt_(m)-S-L-GQDs表面脱落而恢复荧光,进而指示体系中马拉硫磷的检测。本方法的线性范围为5~500μg/L,相关系数R为0.9996,定量限为10.0μg/kg,对结球甘蓝样品中马拉硫磷添加回收率为80.9%~107%,相对标准偏差(RSD)为6.1%~8.9%。研究结果表明O-Aptm荧光探针法可用于马拉硫磷的快速检测,也为其他农药残留的快速检测提供了一种新的手段。This study utilized the principle of Fluorescence Resonance Energy Transfer(FRET)to synthesize a functional graphene fluorescent probe for detecting malathion in cabbage.Functional graphene quantum dots(S-L-GQDs)were obtained via the pyrolysis of a mixture of serine(L-Ser),lysine(L-Lys),and citric acid using a bottom-up approach.These S-L-GQDs were covalently connected to an amino modified malathion aptamer(O-Apt_(m)),forming O-Apt_(m)-S-L-GQDs linkers,exhibiting excellent fluorescence properties.The interaction between graphene oxide(GO)and O-Apt_(m)-S-L-GQDs results in fluorescence quenching.In the presence of the target substance malathion,GO falls off the surface of O-Apt_(m)-S-L-GQDs and restores fluorescence,indicating the detection of malathion in the system.The method demonstrated a linear range of 5-500μg/L,with a correlation coefficient of 0.9996 and a quantification limit of10.0μg/kg.The recovery rate of malathion spiked in cabbage samples ranged from 80.9%to 107%,with relative standard deviations(RSD)between 6.1%and 8.9%.The research results indicate that the O-Apt_(m) fluorescent probe method can be used for rapid detection of malathion,and also provides a promising approach for rapid detection of other pesticide residues.

关 键 词：石墨烯量子点 马拉硫磷 荧光检测 

分 类 号：O657.3[理学—分析化学] TS255.7[理学—化学]