Simulation of cells mechanical responses during perfusion culture in Voronoi-lattice scaffolds using multiphase FSI model  

多相FSI模拟Voronoi-晶格支架灌流式培养中细胞的力学响应

作　　者：Shanshan Zou He Gong Jiazi Gao Liming Zhou 邹姗姗;宫赫;高甲子;周立明

机构地区：[1]Department of Mechanics,College of Mechanical and Aerospace Engineering,Jilin University,Changchun 130022,China [2]School of Biological Science and Medical Engineering,Beihang University,Beijing 100191,China

出　　处：《Acta Mechanica Sinica》2024年第12期40-55,共16页力学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant No.12272029).

摘　　要：During perfusion culture,the growth of bone tissues in the scaffold was closely related to the locations of initial adhered cells and their density.In this study,the fluid mechanical responses of Voronoi-lattice scaffolds and initial adhered cells on scaffolds were quantitatively investigated.Multiphase fluid-structure interaction(FSI)model was verified by comparing with the results of Diamond scaffolds culture in the literature.Fluid mechanical responses of Voronoi-lattice scaffolds and cells were analyzed by multiphase FSI model.Regression equations were established by response surface method(RSM)to determine relationships between structural design factors of Voronoi-lattice scaffolds and fluid mechanical response parameters of scaffolds and cells.The results showed that the percentage of adhered cells and the locations of initial adhered cells obtained by multiphase FSI model of Diamond scaffolds had the same trend with that obtained by perfusion culture.Regression equations established based on RSM could well predict the fluid mechanical response parameters of Voronoi-scaffolds and cells.The multiphase FSI model closely related the densities of cells and the locations of adhered cells to bone tissue growth.The model could provide a certain theoretical basis for constructing and culturing engineered bone tissues in vitro perfusion.在灌流式细胞培养过程中,支架内骨组织的生长与细胞的初始黏附位置及密度密切相关.本文通过定量研究Voronoi-晶格支架及支架内初始黏附细胞的力学响应,为探讨灌流式支架内适宜细胞黏附、增殖和分化的体外力学环境提供理论依据.通过与以往文献中Diamond支架灌流式细胞培养试验结果进行对比,验证多相FSI仿真方法的准确性;采用多相FSI仿真分析Voronoi-晶格支架和细胞的力学响应;最后通过响应面法建立回归模型确定Voronoi晶胞结构设计因素与支架、细胞力学响应参数间关系.Diamond支架多相FSI仿真与灌流式细胞培养试验得到的细胞初始黏附率和黏附位置具有较好的趋势一致性;基于响应面法建立的回归模型很好地预测了Voronoi-晶格支架、细胞的力学响应参数.多相FSI计算模型的使用,将细胞密度及黏附位置分布与骨组织生长紧密连接,为体外灌流构建和培养工程化骨组织提供了一定的理论依据和模拟方法.

关 键 词：Voronoi-lattice scaffold Multiphase flow Fluid-structure interaction Cell trajectory Mechanical response 

分 类 号：Q27[生物学—细胞生物学]