朱鹮β-防御素基因的血液表达谱及相关性分析  

Blood Expression Profile in Blood and Correlation Analysis ofβ-defensin Gene in Crested Ibis(Nippion nippion)

作　　者：邱国强 黄韵怡 俞越 石坚 刘小甜 何珂 方盛国[2] QIU Guoqiang;HUANG Yunyi;YU Yue;SHI Jian;LIU Xiaotian;HE Ke;FANG Shengguo(Deqing County Ecological Forestry Comprehensive Service Center,Deqing 313200,China;MOE Key Laboratory of Biosystems Homeostasis&Protection,State Conservation Centre for Gene Resources of Endangered Wildlife,College of life Sciences,Zhejiang University,Hangzhou,310058,China;College of Animal Science and Technology,College of Veterinary Medicine,Zhejiang A&F University,Hangzhou 311302,China)

机构地区：[1]浙江省德清县生态林业综合服务中心,浙江德清313200 [2]浙江大学生命科学学院,生命系统稳态与保护教育部重点实验室,国家濒危野生动植物种质基因保护中心,浙江杭州310058 [3]浙江农林大学动物科技学院动物医学院,浙江杭州311302

出　　处：《浙江林业科技》2024年第6期1-7,共7页Journal of Zhejiang Forestry Science and Technology

基　　金：浙江省“尖兵”“领雁”研发攻关项目(2023C03138);朱鹮招标项目(ZJXRDQ-2023-DT05)。

摘　　要：由于种群瓶颈效应,出现朱鹮Nippion nippion人工育种受精率低和雏鸟存活率低等现象,从而限制了其重引入工作,亟须从分子层面对朱鹮的免疫情况作进一步研究。防御素是重要的免疫效应分子,在生物体抗菌及适应环境的过程中发挥着重要作用,但目前缺少对朱鹮种群β-防御素基因表达的研究。本研究以浙江德清下渚湖朱鹮繁育基地的74只朱鹮为研究对象,以其外周血为研究样品,通过实时荧光定量PCR检测了朱鹮11个β-防御素基因的血液表达水平,构建了浙江朱鹮种群血液β-防御素基因表达谱,并对广泛表达的AvBD1α、AvBD2、AvBD3、AvBD4及AvBD7基因表达情况进行分析。结果表明:(1)5个广泛表达的AvBD基因相对表达水平在两两之间呈现极显著的正相关(P<0.01);(2)朱鹮幼鸟血液的AvBD的相对表达水平高于亚成体及成鸟,推测其在幼鸟生长发育和适应环境中发挥重要作用;(3)亲代朱鹮与子代朱鹮的AvBD表现出中度至高度的正相关。本研究结果对于筛选具有较强免疫力的朱鹮个体、组建野化放归种群具有指导意义。The reintroduction efforts for the crested ibis(Nipponia nippon)have been hampered by a population bottleneck,leading to low fertility rates and poor chick survival during artificial breeding programs.This highlights the urgent need for molecular-level research into their immune mechanisms.Among these mechanisms,defensins are critical immune effector molecules that play a key role in antibacterial defense and environmental adaptability.However,there is a notable lack of research on the expression of β-defensin genes in crested ibis populations.To address this gap,we investigated β-defensin gene expression using peripheral blood samples from 74 crested ibises housed at the Xiazhuhu Crested Ibis Breeding Center in Deqing,Zhejiang Province.The expression levels of 11 β-defensin genes were measured using real-time fluorescence quantitative PCR,focusing on the widely expressed genes AvBD1α,AvBD2,AvBD3,AvBD4,and AvBD7.The analysis revealed the following key findings:1.The relative expression levels of the five widely expressed AvBD genes were significantly and positively correlated with one another.2.The relative expression levels of AvBD genes were higher in young birds compared to subadults and adults,suggesting that these genes play a crucial role in the growth,development,and environmental adaptation of young ibises.3.The expression levels of AvBD genes showed moderate to high positive correlations between parent and offspring birds.These findings provide valuable insights for identifying individual crested ibises with robust immunity and hold practical significance for forming rewilding populations to support conservation and reintroduction efforts.

关 键 词：Β-防御素 朱鹮 种群 表达谱 

分 类 号：Q953.3[生物学—动物学]