‘蓝天使’冬青杂种胚培养与子代表型变异分析  

Application of the Embryo Culture in Vitro in Cross Breeding of Ilex×meserveae‘Blue Angel’

作　　者：朱淑霞 彭悠悠 楼啸林 王霁 孙丽娜 邱帅 ZHU Shuxia;PENG Youyou;LOU Xiaolin;WANG Ji;SUN Lina;QIU Shuai(Hangzhou Landscaping Incorporated,Hangzhou,310020)

机构地区：[1]杭州市园林绿化股份有限公司,浙江杭州310020

出　　处：《浙江林业科技》2024年第6期40-45,共6页Journal of Zhejiang Forestry Science and Technology

基　　金：浙江省“十四五”花卉新品种选育专项(2021C02071)。

摘　　要：冬青属Ilex植物种质资源丰富,在园林绿化中广泛应用。然而,其种子萌发需2~3 a,十分不利于育种工作的开展。为有效缩短冬青属植物的杂交育种周期,提高种子萌发率,本研究以‘蓝天使’冬青I.×meserveae‘Blue Angel’为母本,3个中国冬青野生种和3个欧洲冬青种(品种)为父本开展人工杂交和不同杂交种子胚培养研究。结果表明:(1)最适合‘蓝天使’冬青离体胚培养的启动培养基是MS培养基;(2)‘蓝天使’冬青与3个中国冬青野生种杂交的坐果率(9.75%~17.16%)低于与3个欧洲冬青种(品种)杂交的坐果率(20.27%~53.30%);(3)离体胚培养可缩短冬青种子萌发时间1~2 a;(4)杂交后代性状与母本‘蓝天使’冬青性状较为接近。本研究不仅为‘蓝天使’冬青种质创制提供技术指导,更为冬青属杂交育种中的胚培养技术研究提供参考。Ilex has rich plant germplasm resources and has been widely planted in landscaping.However,it takes 2~3 years for the seeds to germinate,which is not conducive to breeding.In order to shorten the hybrid breeding cycle of Ilex and improve the seed germination rate,I.×meserveae'Blue Angel'was chosen as the female parent and 6 Ilex species(varieties)were chosen as the male parent for artificial hybridization.The embryos of different hybrid seeds were cultured in vitro.The results showed that:(1)MS medium was the most suitable starting medium for embryo cultures in vitro of I.×meserveae‘Blue Angel’.(2)The fruit setting rates of I.×meserveae‘Blue Angel’crossed with 3 Chinese wild Ilex species were lower(9.75%~17.16%)than those with 3 European Ilex species(varieties)(20.27%~53.30%).(3)The embryo culture in vitro was an effective method to shorten the germination time of Ilex seeds by 1~2 years.(4)The traits of the hybrid offspring are similar to those of the mother I.×meserveae'Blue Angel'.This study not only provided technical guidance for the creation of I.×meserveae‘Blue Angel’germplasm,but also provided reference for the research of embryo culture technology in the cross breeding of Ilex.

关 键 词：冬青属 远缘杂交 杂种胚 组织培养 

分 类 号：S793[农业科学—林木遗传育种]