One-pot synthesis of hydroxyapatite hybrid bioinks for digital light processing 3D printing in bone regeneration  

作　　者：Xiaoxiang Ren Jian Wang Yan Wu Yuan Zhang Jieyuan Zhang Long Bai Jinlong Liu Guangfeng Li Peiran Song Zhongmin Shi Jiacan Su 

机构地区：[1]Institute of Translational Medicine,Shanghai University,Shanghai 200444,China [2]Musculoskeletal Organoid Research Center,Shanghai University,Shanghai 200444,China [3]School of Medicine,Shanghai University,Shanghai 200444,China [4]Department of Orthopedic,Xin Hua Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200092,China [5]National Center for Orthopaedics,Shanghai Sixth People’s Hospital,Shanghai 200233,China [6]Department of Orthopedic Surgery,Shanghai Sixth People’s Hospital,Shanghai 200233,China [7]Department of Trauma Orthopedics,Zhongye Hospital,Shanghai 200941,China

出　　处：《Journal of Materials Science & Technology》2024年第21期84-97,共14页材料科学技术（英文版）

基　　金：financial support from the National Natural Science Foundation of China(Nos.82202335,82230071,and 82172098);the Shanghai Sailing Program(No.22YF1414000).

摘　　要：Three-dimensional(3D)bioprinting has revolutionized tissue engineering by enabling precise fabrication with bioinks.Among these techniques,digital light processing(DLP)stands out due to its exceptional resolution,speed,and biocompatibility.However,the progress of DLP is hindered by the limited availability of suitable bioinks.Currently,some studies involve simple mixing of different materials,resulting in bioinks that lack uniformity and photopolymerization characteristics.To address this challenge,we present an innovative one-pot synthesis method for bioinks based on methacrylated gelatin/alginate with hydroxyapatite(HAP).This approach offers significant advantages in terms of efficiency and uniformity.The synthesized bioinks demonstrate excellent printability,stability,and notably enhanced mechanical properties,facilitating optimal in vitro compatibility.Additionally,the HAP-hybrid bioinks printed scaffolds demonstrated impressive bone repair capabilities in vivo compared with pure organic bioinks.In conclusion,the Gel/Alg/HAP bioinks presented herein offer an innovative solution for DLP bioprinting within the field of bone tissue engineering.Their multifaceted advantages help overcome the limitations of restricted bioink choices,pushing forward the boundaries of bioprinting technology and contributing to the progress of regenerative medicine and tissue engineering.

关 键 词：One-pot strategy Hybrid bioinks DLP Bone regeneration 3D printing 

分 类 号：TP391.73[自动化与计算机技术—计算机应用技术]