EB病毒结构研究进展  

Research progress on the structure of Epstein-Barr virus

作　　者：钟玲[1,2] 王昱霏 杨澄宇 黄洋 何会萍 郑清炳 张晓[1] ZHONG Ling;WANG YuFei;YANG ChengYu;HUANG Yang;HE HuiPing;ZHENG QingBing;ZHANG Xiao(College of Pharmacy,Chongqing Medical University,Chongqing 400016,China;State Key Laboratory of Oncology in South China,Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy,Guangdong Provincial Clinical Research Center for Cancer,Sun Yat-sen University Cancer Center,Guangzhou 510060,China;State Key Laboratory of Vaccines for Infectious Diseases,National Institute of Diagnostics and Vaccine Development in Infectious Diseases,Xiamen University,Xiamen 361005,China;Guangzhou Women and Children’s Medical Center,Guangzhou 510623,China)

机构地区：[1]重庆医科大学药学院,重庆400016 [2]华南恶性肿瘤防治全国重点实验室,广东省鼻咽癌诊治研究重点实验室,广东省恶性肿瘤临床医学研究中心,中山大学肿瘤防治中心,广州510060 [3]厦门大学传染病疫苗研发全国重点实验室,厦门大学国家传染病诊断试剂与疫苗工程技术研究中心,厦门361005 [4]广州市妇女儿童医疗中心,广州510623

出　　处：《中国科学：生命科学》2024年第12期2245-2262,共18页Scientia Sinica(Vitae)

基　　金：国家自然科学基金(批准号:82371832);重庆市自然科学基金(批准号:2023NSCQ-MSX1536);重庆市教育委员会科学技术研究计划(批准号:KJQN202300453)资助。

摘　　要：EB病毒(Epstein-Barr virus,EBV)是一种在人类中广泛传播的γ疱疹病毒,其感染与传染性单核细胞增多症、多种肿瘤、自身免疫病及长新冠等的发生发展息息相关.EBV造成严重的疾病负担,但目前尚无有效的疫苗或特异的抗病毒药物上市.EBV属于双链DNA病毒,具有疱疹病毒典型的包膜-间层-核衣壳三层结构.近年来,随着结构生物学技术的进步,EBV包膜糖蛋白、间层蛋白及核衣壳蛋白的空间结构得到解析,并且蛋白之间的相互作用网络及衣壳蛋白亚基之间的相互作用得到阐明,这将为EBV疫苗、抗体及小分子抑制剂的合理设计提供指导依据.此外,EBV相关蛋白的结构解析将有助于理解病毒与宿主间的相互作用,深入探究病毒蛋白的功能.本文将对EBV结构、病毒组装与释放过程、目前已报道的EBV包膜糖蛋白、间层蛋白及核衣壳蛋白的结构与功能以及这三类蛋白间的相互作用网络进行综述,旨在为以结构为基础的疫苗及特异性小分子药物设计等提供理论基础.Epstein-Barr virus(EBV),a humanγherpes virus,is a widely spread worldwide.EBV infection is closely related to the occurrence and development of infectious mononucleosis,various tumors,autoimmune diseases and Long COVID.EBV causes a serious disease burden,but there is currently no effective vaccines or specific antiviral drugs on the market.EBV belongs to the double stranded DNA virus and has a typical three-layer structure of envelope interlayer nucleocapsid of herpes virus.In recent years,with the advancement of structural biology technology,the spatial structures of EBV envelope glycoproteins,interlayer proteins,and nucleocapsid proteins have been elucidated,and the interaction networks between proteins and the interactions between capsid protein subunits have also been demonstrated.This will provide guidance for the rational design of EBV vaccines,antibodies,and small molecule inhibitors.In addition,structural analysis of EBV-encoded proteins will aid to understand the interaction between the virus and the host,and further explore the function of viral proteins.This review article summarizes the structure of EBV,virus assembly and release processes,the structures and functions of EBV envelope glycoproteins,interlayer proteins,and nucleocapsid proteins that have been reported so far,as well as the interaction networks between these three types of proteins.These findings will pave the way for the rational design of vaccines and therapeutics on the basis of EBV structures against EBV infection and associated diseases.

关 键 词：EB病毒 包膜糖蛋白 间层蛋白 核衣壳蛋白 抗病毒策略 

分 类 号：R373[医药卫生—病原生物学]