不同氢分压对氨氮和CO_(2)同步合成微生物蛋白的影响  

Effects of different hydrogen partial pressure on microbial protein production via simultaneous conversion of CO_(2)and ammonia

作　　者：肖祥杰 陈进 吴和瀛 杨紫怡 XIAO Xiangjie;CHEN Jin;WU Heying;YANG Ziyi(Beijing University of Chemical Technology,College of Chemical Engineering,Beijing 100029,China;Beijing Deliangyuan Biotechnology Co.Ltd,Beijing 100081,China)

机构地区：[1]北京化工大学化学工程学院,北京100029 [2]北京德量源生物科技有限公司,北京100081

出　　处：《环境工程学报》2024年第10期2939-2946,共8页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金资助项目(52100023,52270096);国家自然科学基金企业创新发展联合基金资助重点项目(U20B2022);新疆生产建设兵团科技计划项目(2021DB006)。

摘　　要：氢氧化细菌(HOB)可同步实现氨氮转化和CO_(2)固定并产生微生物蛋白,具有广泛的应用价值。本研究探究不同氢分压对HOB体系内CO_(2)固定、氨氮转化以及产蛋白过程的影响。结果表明,HOB体系的性能随氢分压的增大而加强。氢分压为0.8 bar时,细胞密度(OD_(620))和RuBP羧化酶浓度达到最大(OD_(620)=0.50;酶浓度为(41.27±1.20)ng·L^(-1)),同时CO_(2)固定速率和氨氮转化率最高,分别为0.06L·(L·d)^(-1)和68.48%。当氢分压较高时(≥0.8 bar),HOB产微生物蛋白的能力得到显著增强,蛋白产量高于1.3 g·L^(-1),蛋白含量高于68%,且氨基酸产量高于950 mg·L^(-1)。通过关键因子的相关性分析,氢分压与HOB体系内CO_(2)固定速率、H2消耗速率、细胞干质量、粗蛋白含量的相关性显著(R^(2)>0.8),与氨氮转化率之间存在显著的二次关系(R^(2)=0.99)。因此,利用HOB合成微生物蛋白的过程中,可通过适当增加氢分压来优化系统运行,从而为后续高效、高质合成微生物蛋白的应用提供数据支持。Hydrogen-oxidizing bacteria(HOB)can simultaneously convert ammonia and CO_(2)into microbial protein(MP),which has a great potential for application.This study investigated the effects of different hydrogen partial pressures on CO_(2)fixation,ammonia conversion and protein production in HOB system.The results showed that the HOB system was strengthened with the increment of hydrogen partial pressure.At the hydrogen partial pressure of 0.8 bar,the cell density(OD_(620))and the concentration of RuBP carboxylase reached the maximum vaules of OD_(620)=0.50,concentration of enzyme was((41.27±1.20)ng·L^(-1),respectively.Meanwhile,the CO_(2)fixation rate and ammonia conversion rate also reached the highest values of 0.06 L·(L·d)^(-1)and 68.48%,respectively.At the hydrogen partial pressure was equal to or higher than 0.8 bar,the capacity of HOB to produce MP was significantly enhanced,and the protein yield,protein content and amino acid yield were higher than 1.3 g·L^(-1),68%,and 950 mg·L^(-1),respectively.The correlation analysis among the key factors indicated the significant linear correlations occurred between hydrogen partial pressure and CO_(2)fixation rate,H2 consumption rate,cell dry weight,or crude protein content in HOB system(R^(2)>0.8),and a significant quadratic relationship occurred between hydrogen partial pressure and ammonia conversion rate(R^(2)=0.99).Therefore,the system operation can be optimized through appropriately increasing the hydrogen partial pressure in the process of MP production via HOB.This study could provide data support and theoretical foundation for the high efficiency and quality of MP production in practical application.

关 键 词：氢氧化细菌 氢分压 CO_(2)固定 微生物蛋白 

分 类 号：X703[环境科学与工程—环境工程]