2020—2023年沧州市食源性沙门菌分子分型及药敏结果分析  

Analysis of molecular classification and drug susceptibility results of food-borne Salmonella in Cangzhou City from 2020 to 2023

作　　者：卢伟 戴其彬 胡倩倩 关树培 王春东[1] LU Wei;DAI Qibin;HU Qianqian;GUAN Shupei;WANG Chundong(Microbial Laboratory Department,Cangzhou Center for Disease Control and Prevention,Cangzhou,Hebei 061000,China)

机构地区：[1]沧州市疾病预防控制中心微生物检验科,河北沧州061000

出　　处：《职业与健康》2024年第21期2909-2912,共4页Occupation and Health

基　　金：沧州市科技计划自筹经费项目(222106013)。

摘　　要：目的了解沧州市食源性沙门菌的污染状况,探讨其分子分型及药物敏感性情况,为预防食源性沙门菌病提供科学依据。方法2020—2023年,对分离出的88株沙门菌进行分子分型,包括血清分型和脉冲场凝胶电泳分型(pulsed field gel electrophoresis PFGE);利用药敏分析系统对菌株进行耐药监测。结果88株沙门菌血清型和PFGE条带均呈多样性,共检出22种血清型,其中肠炎沙门菌和鼠伤寒沙门菌分别占30.68%和23.86%,为本地主导血清型;PFGE分型获得81种条带,聚类分析相似度为53.2%~100.0%,优势条带包含10株菌。88株沙门菌对不同抗生素的耐药性差异有统计学意义(P<0.05),多数菌株药物敏感性差,耐药严重,多重耐药的菌株占比高达52.27%,氨苄西林、氨苄舒、四环素耐药率均超过50%。结论沧州市沙门菌血清型分布广泛,优势血清型为肠炎沙门菌和鼠伤寒沙门菌。PFGE条带多样,同一血清型大部分菌株PFGE型可聚集成簇,大部分菌株多重耐药,耐药问题较为严重。Objective To understand the contamination status of food-borne Salmonella in Cangzhou City,explore its molecular classification and drug susceptibility,and provide scientific basis for the prevention of food-borne Salmonella diseases.Methods From 2020 to 2023,88 strains of Salmonella were typed by molecular typing,including serum typing and pulsed field gel electrophoresis(PFGE)typing,and drug resistance of strains was monitored by drug susceptibility analysis system.Results The serotypes and PFGE bands of 88 Salmonella strains showed diversity,with a total of 22 serotypes detected.Among them,Salmonella enterica and Salmonella typhimurium accounted for 30.68% and 23.86%,respectively,and were the dominant serotypes in the local area.81 bands were obtained by PFGE typing,the similarity of cluster analysis was 53.2%-100.0%,and the dominant bands included 10 strains.There was a statistically significant difference in the resistance of 88 strains of Salmonella to different antibiotics(P<0.05).Most of the strains had poor drug sensitivity and severe drug resistance.The proportion of multi-drug resistant strains was as high as 52.27%,the resistance rates of ampicillin,ampicillin and tetracycline were all more than 50%.Conclusion Salmonella serotypes are widely distributed in Cangzhou City,with predominant serotypes being Salmonella enterica and Salmonella typhimurium.PFGE bands are diverse,and the PFGE types of most strains of the same serotype can cluster together.Most strains are multidrug-resistant,and the problem of drug resistance is quite serious.

关 键 词：沙门菌 分子分型 药敏结果分析 脉冲场凝胶电泳 多重耐药 

分 类 号：R117[医药卫生—公共卫生与预防医学]