超敏蛋白HrpZ的可溶性表达条件研究进展  

Research progress on soluble expression conditions of hypersensitive protein HrpZ

作　　者：赵艺博 彭灿明 王娟萍 刘森 ZHAO Yibo;PENG Canming;WANG Juanping;LIU Sen(Collaborative Innovation Center of Industrial Fermentation(Ministry of Education&Hubei Province),Hubei University of Technology,Wuhan 430068;Key Laboratory of Fermentation Engineering(Ministry of Education),Hubei University of Technology,Wuhan 430068;Hubei WEL-SAFE Biotechnology Co.,Ltd.,Ezhou 436006,China)

机构地区：[1]湖北工业大学工业发酵省部共建协同创新中心,湖北武汉430068 [2]湖北工业大学发酵工程教育部重点实验室,湖北武汉430068 [3]湖北微生元生物科技有限公司,湖北鄂州436006

出　　处：《生物技术》2024年第6期780-787,共8页Biotechnology

基　　金：国家自然科学基金项目(31971150);湖北省杰出青年基金项目(2019CFA069);工业发酵省部共建协同创新中心开放基金。

摘　　要：该文综述了超敏蛋白HrpZ在植物病害防治中的重要性及其在不同治病变种中的可溶性表达条件。特别关注HrpZ在植物免疫和促进植物生长抗病性方面的作用。总结了针对不同变种的可溶性表达条件的研究进展,并探讨了HrpZ在工业生产中的挑战。对蛋白折叠因子和序列优化等思考,展望了未来研究方向和纯化方法的改进,旨在推动HrpZ蛋白在植物病害防治领域的应用。The importance of hypersensitivity protein HrpZ in plant disease control and its soluble expression conditions in dif-ferent treated species were reviewed.Particular attention is paid to the role of HrpZ in plant immunity and promoting plant growth and disease resistance.This paper summarizes the research progress on soluble expression conditions for different vari-ants,and discusses the challenges of HrpZ in industrial production.This paper puts forward questions and reflections,including protein folding factors and sequence optimization,and looks forward to future research directions and improvement of purification methods,aiming to promote the application of HrpZ protein in the field of plant disease control.

关 键 词：HrpZ蛋白 超敏反应 植物免疫 生物农药 外源表达 包涵体 分离纯化 工业应用 

分 类 号：Q939.96[生物学—微生物学]