基于UPLC-Q-Exactive Orbitrap-MS技术的竹节参提取物血清药物化学研究  

Serum pharmacochemistry study of Panacis Japonici Rhizoma extract based on UPLC-Q-Exactive Orbitrap-MS

作　　者：温媛 刘畅[1,2] 原琪 王锐[1,3] 李雪晴 袁丁[1,2] 袁成福 何毓敏[1,2] WEN Yuan;LIU Chang;YUAN Qi;WANG Rui;LI Xue-qing;YUAN Ding;YUAN Cheng-fu;HE Yu-min(Third-grade Pharmacological Laboratory on Traditional Chinese Medicine,State Administration of Traditional Chinese Medicine,China Three Gorges University,Yichang 443002,China;College of Medicine and Health Science,China Three Gorges University,Yichang 443002,China;College of Basic Medical Sciences,China Three Gorges University,Yichang 443002,China)

机构地区：[1]三峡大学国家中医药管理局药理科研三级实验室,湖北宜昌443002 [2]三峡大学健康医学院,湖北宜昌443002 [3]三峡大学基础医学院,湖北宜昌443002

出　　处：《中国中药杂志》2024年第23期6490-6499,共10页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(82374107,82174035,81974528,81773959);湖北省自然科学基金创新群体项目(2021CFA015);湖北省中央引导地方科技发展专项(2020ZYYD016)。

摘　　要：该研究运用超高效液相色谱-四极杆静电场轨道阱质谱(UPLC-Q-Exactive Orbitrap-MS)技术对竹节参提取物及其入血成分进行分析。采用Waters UPLC BEH C_(18)色谱柱(2.1 mm×100 mm,1.7μm),0.1%甲酸水溶液-0.1%甲酸乙腈为流动相,梯度洗脱,电喷雾离子源正、负离子模式下得到高分辨多级质谱数据后与对照品保留时间、高分辨精确相对分子质量、二级碎片信息及相关文献比对,阐明竹节参提取物化学成分。通过大鼠灌胃给予竹节参提取物后腹主动脉取血分离血清,扫描鉴定入血成分。最终在竹节参提取物中检测出43个化学成分,包括22个三萜皂苷类、9个氨基酸类、5个多糖类、2个挥发油类、5个核苷类。在竹节参提取物含药血清中检测出18个入血成分,包括10个原型成分、6个代谢产物和2个未知成分。该研究分析了竹节参提取物及入血成分,为明确竹节参的药效物质基础提供线索。In this study,chemical components of Panacis Japonici Rhizoma extract and absorbed components in rat were identified by ultra-high performance liquid chromatography-quadrupole electrostatic field orbital trap mass spectrometry(UPLC-Q-Exactive Orbitrap-MS).The separation was performed on Waters UPLC BEH C_(18) column(2.1 mm×100 mm,1.7μm)with mobile phase gradient elution of water and acetonitrile containing 0.1%formic acid,high resolution multistage mass spectrometry data were collected by electrospray ionization in positive and negative ion modes.The chemical composition of Panacis Japonici Rhizoma extract were identified by comparing with the retention time,high resolution precise molecular weight,secondary fragment ions of reference substance and related literature.After intragastric administration of Panacis Japonici Rhizoma extract,blood was collected from the abdominal aorta of rats to separate serum,and the absorbed components were scanned and identified.Finally,43 chemical components were detected in the Panacis Japonici Rhizoma extract, including 22 saponins, 9 amino acids, 5 polysaccharides, 2 volatile oils and 5 nucleosides. In serum, 18 componentswere detected, including 10 prototype components, 6 metabolites and 2 unknown components. The study ana lyzed the PanacisJaponici Rhizoma extract and serum containing Panacis Japonici Rhizoma components, for clarifying the pharmacologicalbasis of Panacis Japonici Rhizoma to provide reference.

关 键 词：竹节参提取物 血清药物化学 原型成分 代谢产物 UPLC-Q-Exactive Orbitrap-MS 

分 类 号：R284.1[医药卫生—中药学]