手性金属-有机框架二维材料的液相色谱拆分性能研究  

Resolution Characteristic of Two Chiral Two-Dimensional Metal-Organic Frameworks in Liquid Chromatography

作　　者：王雪萍 高顺秋 李毅洁 杨梅芳 龙怡幸 袁黎明[1] WANG Xue-ping;GAO Shun-qiu;LI Yi-jie;YANG Mei-fang;LONG Yi-xing;YUAN Li-ming(School of Chemistry and Chemical Engineering,Yunnan Normal University,Kunming 650500,China)

机构地区：[1]云南师范大学化学化工学院,云南昆明650500

出　　处：《化学试剂》2025年第1期17-25,共9页Chemical Reagents

基　　金：基于二维材料的薄片型气相色谱柱手性柱研究项目(22174125)。

摘　　要：二维金属-有机框架材(2D-MOFs)具有大比表面积、厚度小、活性位点充分暴露、传质阻力小等优点,将其用作高效液相色谱(HPLC)固定相理论上可以增加色谱柱的塔板数,进而提高其分离效能,在手性分离领域展现出独特的应用价值。将手性分子L-羟基脯氨酸和D-(+)-葡萄糖分别修饰到NH_(2)-Ni/Co MOFs和Zn-NH_(2)-BDC MOFs两种2D-MOFs上,制备二维手性金属-有机框架纳米片(2D-CMOFs),再将Zn-NH_(2)-BDC-D-(+)-葡萄糖和NH_(2)-Ni/Co-L-羟基脯氨酸通过“网包法”固载在硅胶表面制备手性固定相。实验表明:19种手性药物和5种位置异构体被NH_(2)-Ni/Co-L-羟基脯氨酸手性色谱柱拆分开,基线分离了6种手性药物和3种位置异构体;10种手性药物和3种位置异构体被Zn-NH_(2)-BDC-D-(+)-葡萄糖色手性谱柱拆分开;且两个手性柱都具有良好的重复性。Two-dimensional metal-organic frame materials(2D-MOFs)have the advantages of large specific surface area,small thickness,full exposure of active sites,and minimal mass transfer resistance.Their application in high-performance liquid chromatography(HPLC)can theoretically increase the number of column plates and improve its separation efficiency,showing unique application value in the chiral separation field.Two-dimensional chiral metal-organic framework nanosheets(2D-CMOFs)were prepared by modifying the chiral molecules L-hydroxyproline and D-(+)-glucose onto NH_(2)-Ni/Co MOFs and Zn-NH_(2)-BDC MOFs,respectively.The chiral stationary phase was created by applying NH_(2)-Ni/Co-L-hydroxyproline and Zn-NH_(2)-BDC-D-(+)-glucose onto the silica gel surface using the“mesh coating method”.The results showed that 19 chiral drugs and 5 site isomers were separated by NH_(2)-Ni/Co-L-hydroxyproline chiral column,and 6 chiral drugs and 3 site isomers were baseline separated.Ten chiral drugs and three site isomers were separated by Zn-NH_(2)-BDC-D-(+)-glucose-colored chiral spectrum.Both chiral columns have good repeatability.

关 键 词：手性分离 高效液相色谱 二维手性金属-有机框架材料 手性固定相 手性化合物 

分 类 号：O657.7[理学—分析化学]