多种金属有机框架材料的荧光淬灭-恢复性能研究  

Study of Fluorescence Quenching-Recovery Properties of a Variety of Metal-Organic Framework Materials

作　　者：徐奥 韦宇新 施润呈 孙璇 XU Ao;WEI Yu-xin;SHI Run-cheng;SUN Xuan(School of Basic Medical Sciences,Hubei University of Chinese Medicine,Wuhan 430056,China)

机构地区：[1]湖北中医药大学基础医学院,湖北武汉430056

出　　处：《化学试剂》2025年第1期33-38,共6页Chemical Reagents

基　　金：湖北省教育厅科学技术研究项目(Q20232008);湖北省自然科学基金项目(2024AFB375);湖北中医药大学博士启动经费项目(2023ZXB015)。

摘　　要：金属有机框架(Metal-organic frameworks,MOFs)作为一种新型多孔晶体材料受到广泛关注,旨在研究其作为生物传感原件中的荧光淬灭-恢复性能。ZIF-8、MIL-101(Fe)、MIL-101(Cr)、UIO-66、2D-pMOFs 5种MOFs材料分别采用溶剂热法进行化学合成,然后分别与荧光探针、互补DNA孵育研究其荧光强度变化,并测定反应液在485 nm激发波长下的荧光强度值。ZIF-8、MIL-101(Fe)、MIL-101(Cr)几乎无荧光淬灭-恢复活性,2D-pMOFs具有一定的荧光淬灭-恢复效率,UIO-66的荧光淬灭-恢复效率最强。UIO-66的荧光淬灭特性源于金属离子锆的光诱导电子转移效应;而荧光恢复能力来自其结构上的多孔性。UIO-66通过对DNA的吸附/解吸实现DNA探针的荧光淬灭/恢复,在生物传感领域显示出巨大的应用潜力。Metal-Organic Frameworks(MOFs)has attracted widespread attention as a new type of porous crystal material.The aim of this study is to investigate its fluorescence quenching-recovery performance as a biosensor.ZIF-8,MIL-101(Fe),MIL-101(Cr),UIO-66,and 2D-pMOFs were synthesized by solvothermal method,and then incubated them with fluorescent probes and complementary DNA to study their fluorescence intensity changes.ZIF-8,MIL-101(Fe)and MIL-101(Cr)had almost no fluorescence quenching-recovery activity,2D-pMOFs had certain fluorescence quenching-recovery efficiency,and UIO-66 had the strongest fluorescence quenching-recovery efficiency.The fluorescence quenching properties of UIO-66 were derived from the photoinduced electron transfer(PET)effect of zirconium metal ions.The fluorescence recovery ability comed from its structural porosity.UIO-66 realized fluorescence quenching/recovery of DNA probes through adsorption/desorption of DNA,and showed great application potential in the field of biosensor.

关 键 词：金属有机框架(MOFs) 荧光淬灭 荧光恢复 荧光探针 纳米材料 

分 类 号：O65[理学—分析化学]