共同固定化漆酶介体高效降解磺胺甲恶唑  

Efficient Degradation of Sulfamethoxazole by Co-Immobilized Laccase Mediator

作　　者：周立强 马骏 黄大伟 ZHOU Liqiang;MA Jun;HANG Dawei(South China Institute of Environmental Sciences,Ministry of Ecology and Environment(Research Institute of Eco-environmental Emergency,Ministry of Ecology and Environment),Guangzhou 510530,China;Yunnan Ecological Environment Emergency Investigation and Complaint Center,Kunming 650032,China)

机构地区：[1]生态环境部华南环境科学研究所(生态环境部生态环境应急研究所),广东广州510530 [2]云南省生态环境应急调查投诉中心,云南昆明650032

出　　处：《水处理技术》2024年第12期22-27,共6页Technology of Water Treatment

基　　金：国家重点研发计划(2022YFC3705004)。

摘　　要：鉴于水环境中抗生素的污染问题,设计并开发了一种利用沸石咪唑框架-8(ZIF-8)共同固定化漆酶和丁香醇的固定化漆酶介体系统。采用正交设计方法优化了LMS@ZIF-8的合成条件,并通过多种表征技术验证了该材料的结构与功能。实验结果显示,与游离漆酶相比,LMS@ZIF-8在磺胺甲恶唑(SMX)的降解过程中显著提高了效率。在重复使用性测试中,LMS@ZIF-8即使经多次循环使用仍保持高达90.9%的酶活性。此外,LMS@ZIF-8的合成和应用过程中减少了小分子介体和漆酶的直接使用,有助于降低环境污染和成本。本研究不仅揭示了基于MOF的固定化漆酶系统在水处理领域的潜在应用前景,还为漆酶在环境治理中的实际应用开辟了新策略。Given the pollution issue of antibiotics in the aquatic environment,an immobilized laccase mediator system(LMS)utilizing zeolitic imidazolate framework-8(ZIF-8)to co-immobilized laccase and syringyl alcohol has been designed and developed.The synthesis conditions for LMS@ZIF-8 were optimized using the orthogonal design method,and the structure and functionality of the material were verified through various characterization techniques.Experimental results showed that LMS@ZIF-8 significantly enhanced the efficiency of sulfamethoxazole(SMX)degradation compared to free laccase.In the reusability tests,LMS@ZIF-8 maintained up to 90.9%enzymatic activity even after multiple cycles of use.Moreover,the synthesis and application of LMS@ZIF-8 reduced the direct use of small molecular mediators and laccase,which helps to decrease environmental pollution and cost.This study not only reveals the potential application prospects of MOF-based immobilized laccase systems in water treatment but also paves new strategies for the practical application of laccase in environmental management.

关 键 词：漆酶 介体 生物降解 磺胺甲恶唑 金属有机框架 

分 类 号：TB333[一般工业技术—材料科学与工程] X552[环境科学与工程—环境工程]