Co-MOF@Ag NPs/DNA适配体荧光探针选择性检测农产品中痕量噻虫胺残留研究  

The Selective Detection of Trace Clothianidin Residues in Agricultural Products Using Co-MOF@Ag NPs/DNA Aptamer Fluorescent Probes

作　　者：袁维维 黎舒怀[1,2] 马雄辉 庞朝海 王明月 韩丙军[1,2] 李备 YUAN Weiwei;LI Shuhuai;MA Xionghui;PANG Chaohai;WANG Mingyue;HAN Bingjun;LI Bei(Analysis and Testing Center Chinese Academy,Chinese Academy of Tropical Agricultural Sciences/Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables,Haikou,Hainan 571101;National Key Laboratory of Market Regulation,Food Inspection and Testing Center,Hainan Institute of Inspection and Testing(Quality and Safety of Tropical Fruits and Vegetables),Haikou,Hainan 571101;College of Food Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070)

机构地区：[1]中国热带农业科学院分析测试中心/海南省热带果蔬质量安全重点实验室,海南海口571101 [2]海南省检验检测研究院食品检验检测中心国家市场监管重点实验室(热带果蔬质量与安全),海南海口570314 [3]华中农业大学食品科技学院,湖北武汉430070

出　　处：《热带农业工程》2024年第6期25-32,共8页Tropical Agricultural Engineering

基　　金：国家市场监管重点实验室(热带果蔬质量与安全)课题(No.ZX2023002);国家香蕉产业技术体系(No.CARS-31);中国热带农业科学院国家热带农业科学中心科技创新团队(No.CATASCXTD202314)。

摘　　要：荧光探针的选择性识别能力好坏和灵敏度高低决定了荧光检测技术的优劣。本研究以Co为金属源,通过水热法结合辅助配体合成新型Co-MOF@Ag NPs荧光材料,并引入噻虫胺的DNA适配体作为识别元件,构建Co-MOF@Ag NPs/DNA适配体荧光探针。当探针通过DNA适配体捕获样品中噻虫胺,以自适应方式折叠成特定构象,导致Co-MOF@Ag NPs/DNA适配体的荧光信号降低,以此建立检测农产品中噻虫胺残留的新方法。由于探针优异的荧光性能,该方法具有很高的灵敏度;同时,DNA适配体能够特异性识别噻虫胺,有效排除其它结构相似的农药干扰,具有很高的选择性。方法的线性范围为5×10^(−13)~8000×10^(−13)mol/L,检出限为1.67×10^(−13) mol/L。该方法可应用于香蕉、豇豆等实际样品的检测,加标回收率为84.0%~108.2%。The selective recognition ability and sensitivity of fluorescent probes determine the quality of fluorescence detection technology.In this study,a Co-MOF@Ag NPs fluorescent material was synthesized via hydro-thermal method with auxiliary ligand,and then DNA aptamer was introduced as the recognition element to construct Co-MOF@Ag NPs/DNA aptamer fluorescent probe.The probe has excellent fluorescence luminescence,folding in a specific conformation when the probe can capture the DNA aptamer,resulting in a quenching fluorescence intensity of Co-MOF@Ag NPs/DNA aptamer,thus establishing a new method for detecting clothianidin residue in agricultural products.Due to the excellent fluorescence performance of the probe,this method has high sensitivity;Meanwhile,DNA aptamers can specifically recognize clothianidin,which can effectively eliminate other pesticides with similar structure,and has high selectivity.The linear range of the method was 5×10^(−13)mol/L~8000×10^(−13)mol/L,and the detection limit was 1.67×10^(−13) mol/L.This method can be applied to the detection of actual samples such as bananas and cowpeas,with spiked recovery rate ranging from 84.0%-108.2%.

关 键 词：金属有机框架材料 银纳米 DNA适配体 噻虫胺 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]