1株野生黏小奥德蘑的鉴定及生物学特性研究  

Study on the Identification and Biological Characteristics of a Wild Oudemansiella mucida

作　　者：郑永德[1] 张君丽[2] ZHENG Yongde;ZHANG Junli(Putian Institute of Agricultural Sciences,Putian,Fujian 351100;Vegetable Research Institute,Xizang Academy of Agriculture and Animal Husbandry Sciences,Lhasa,Xizang 850000,China)

机构地区：[1]莆田市农业科学研究所,福建莆田351100 [2]西藏自治区农牧科学院蔬菜研究所,西藏拉萨850000

出　　处：《贵州农业科学》2024年第12期50-56,共7页Guizhou Agricultural Sciences

基　　金：特色食用菌种质资源创新研究与菌种繁育关键技术集成示范项目(XZ202001ZY0041N)。

摘　　要：【目的】2021—2022年在西藏地区采集到1株野生菌株,编号为X21198,为该菌株的后续开发利用奠定基础。【方法】应用形态学鉴定与ITS鉴定分析,鉴定野生菌株X21198种性,研究其在不同碳源(葡萄糖、蔗糖、果糖、麦芽糖、甘露糖、淀粉)、氮源(尿素、酵母粉、硫酸铵、牛肉粉、蛋白胨、硝酸铵)、pH(5.0、6.0、7.0、8.0、9.0、10.0)、温度(15℃、20℃、25℃、30℃、35℃、40℃)条件下菌丝的生物学特性。【结果】野生菌株X21198菌盖半球形至平展,黏滑或胶黏,白色,边缘具稀疏而不明显条纹。菌褶直生至弯生,较稀,不等长。菌柄圆柱形,实心,纤维质。菌肉薄,白色。经ITS鉴定,ITS序列与LC149596.1 Mucidula和OP328319.1的相似度为84%,分属为同一类群,确定该菌株为黏小奥德蘑。该菌株培养的最适碳源、氮源、pH和温度分别为果糖、酵母粉、8和25℃。【结论】西藏地区野生菌株X21198为黏小奥德蘑,菌丝培养的最适碳源、氮源、pH和温度分别为果糖、酵母粉、8和25℃。【Objective】A wild fungal strain was collected in Xizang area during 2021—2022 and numbered X21198,which laid the foundation for the subsequent development and utilization of the strain.【Method】The species characteristics of wild strain X21198 were determined by using morphological identification and ITS identification analysis,and the biological characteristics of mycelia were studied under the conditions of different carbon source(glucose,sucrose,fructose,maltose,mannose and starch),nitrogen source(urea,yeast powder,ammonium sulfate,beef meal,peptone and ammonium nitrate),pH(5.0,6.0,7.0,8.0,9.0 and 10.0) and temperature(15 ℃,20 ℃,25 ℃,30 ℃,35 ℃ and 40 ℃).【Result】The cap of wild strain X21198 was hemispherical to flat,slimy or sticky,white and edge with sparse but not distinct stripes.The gill was straight to curved,sparse and unequal long.The stipe was cylindrical,solid and fibrous.The context was thin and white.The similarity of ITS sequence between X21198 and LC149596.1 Mucidula and OP328319.1 was 84%,belonging to the same group,and then the strain is identified as Oudemansiella mucida.The optimal carbon source,nitrogen source,pH and temperature was respectively fructose,yeast powder,8 and 25 ℃.【Conclusion】The wild strain X21198 in Xizang area was identified as O.mucida.The optimal carbon source,nitrogen source,pH and temperature for the mycelia culture is respectively fructose,yeast powder,8 and 25 ℃.

关 键 词：黏小奥德蘑 野生菌 食药用真菌 形态学鉴定 分子鉴定 生物学特性 

分 类 号：S646[农业科学—蔬菜学]