三种单胞藻粉在室内卤虫养殖中的应用  

Application of Three Stains of Microalgae Powder in Indoor Artemia Culture

作　　者：迟庆雷 高美荣 加晶 龙菲平 吴星宇 隋丽英 CHI Qinglei;GAO Meirong;JIA Jing;LONG Feiping;WU Xingyu;SUI Liying(SDIC Biotechnology Investment Co.,Ltd.,Beijing 100034,China;Beijing Key Laboratory of Algae Biomass,Beijing 100142,China;College of Marine and Environmental Sciences,Tianjin University of Science&Technology,Tianjin 300457,China)

机构地区：[1]国投生物科技投资有限公司,北京100034 [2]微藻生物能源与资源北京市重点实验室,北京100142 [3]天津科技大学海洋与环境学院,天津300457

出　　处：《天津科技大学学报》2024年第6期30-37,共8页Journal of Tianjin University of Science & Technology

基　　金：国家外国专家人才类项目(DL2021002001L)。

摘　　要：以三角褐指藻(Phaeodactylum tricornutum)、湛江等鞭金藻(Isochrysis zhanjianggensis)和佐夫色绿藻(Chromochloris zofingiensis)干粉为研究对象,探索合适的卤虫养殖条件,评价饵料单胞藻对卤虫营养和相关酶活性的影响。研究结果明确了三角褐指藻和湛江等鞭金藻的最适投喂量,且盐度70较盐度30有利于卤虫存活,低投放密度(0.1 L^(-1))组卤虫的存活率和体长均大于高投放密度(0.5 L^(-1))组。在初始密度0.1 L^(-1)和盐度70条件下进行40 L水体卤虫养殖,发现投喂佐夫色绿藻组卤虫的存活率和体长均显著高于投喂三角褐指藻和湛江等鞭金藻组。卤虫的营养组成(以干质量计)与饵料单胞藻的营养密切相关,投喂佐夫色绿藻组卤虫粗脂肪含量较高(15.9%)、粗蛋白含量较低(50.44%),而投喂三角褐指藻和湛江等鞭金藻的卤虫粗蛋白含量较高(分别为60.22%和60.91%)、粗脂肪含量较低(分别为4.39%和4.62%)。投喂三角褐指藻组卤虫的二十碳五烯酸(EPA)含量最高(9.47 mg/g),投喂湛江等鞭金藻组卤虫二十二碳六烯酸(DHA)含量最高(0.3 mg/g)。投喂佐夫色绿藻的卤虫总抗氧化能力、总超氧化物歧化酶活性、酸性磷酸酶活性和碱性磷酸酶活性高于其他组。In our current study,three commercially produced microalgae powder Phaeodactylum tricornutum,Isochrysis zhanjianggensis and Chromochloris zofingiensis were applied to Artemia culture,to study the optimum culture conditions and evaluate the effect of microalgae on the nutrition quality and enzyme activity of the Artemia biomass.The study result clarified the optimal feeding level for P.tricornutum and I.zhanjianggensis.It also showed that 70 salinity resulted higher survival than that at 30 salinity,and survival rate and body length of Artemia with low stocking density(0.1 L^(-1))were higher than those with high stocking density(0.5 L^(-1)).When Artemia were cultured at salinity 70 and stocking density 0.1/L in 40 L volume,C.zofingiensis feeding resulted significantly higher Artemia survival rate and body length than other two microalgae feeding.The nutritional composition of Artemia(in terms of dry mass)were closely related to those of microalgae.The crude fat content of Artemia fed with C.zofingiensis had much higher lipid content(15.9%)and lower crude protein content(50.44%),when compared to those fed with P.tricornutum and I.zhanjianggensis(4.39% and 4.62% for crude fat content,60.22% and 60.91% for protein content,respectively).Artemia fed with P.tricornutum had the highest eicosapentaenoic acid(EPA)content(9.47 mg/g),while Artemia fed with I.zhanjianggensis had the highest docosahexoenoic acid(DHA)content(0.3 mg/g).Moreover,Artemia fed with C.zofingiensis also resulted the highest total antioxidant capacity,total superoxide dismutase,acid phosphatases and alkaline phosphatase activities of Artemia.

关 键 词：单胞藻 卤虫养殖 高度不饱和脂肪酸 

分 类 号：S963.21[农业科学—水产养殖]