大麦黄条点花叶病毒致病性及流行风险  

Pathogenicity and Epidemic Risk of Barley Yellow Striate Mosaic Virus

作　　者：张爱红[1] 杨菲[1] 赵元业 赵怡涵 邸垫平[1] 苗洪芹[1] ZHANG AiHong;YANG Fei;ZHAO YuanYe;ZHAO YiHan;DI DianPing;MIAO HongQin(Plant Protection Institute,Hebei Academy of Agriculture and Forestry Sciences/IPM Innovation Center of Hebei Province/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China,Ministry of Agriculture and Rural Affairs/International Science and Technology Joint Research Center on IPM of Hebei Province,Baoding 071000,Hebei)

机构地区：[1]河北省农林科学院植物保护研究所/河北省农业有害生物综合防治技术创新中心/农业农村部华北北部作物有害生物综合治理重点实验室/河北省作物有害生物综合防治国际科技联合研究中心,河北保定071000

出　　处：《中国农业科学》2024年第23期4686-4697,共12页Scientia Agricultura Sinica

基　　金：河北省现代农业产业技术体系建设专项资金(HBCT 2023100210);河北省农林科学院现代农业科技创新专项(2022KJCXZX-ZBS-10)。

摘　　要：【目的】大麦黄条点花叶病毒(barley yellow striate mosaic virus,BYSMV)在我国北方麦区分布广泛,研究明确BYSMV对主要作物的致病性以及田间自然寄主,评估病害在禾本科作物种植区的流行风险,为该病害的精准防控提供科学依据。【方法】利用携带BYSMV的灰飞虱将病毒人工接种于小麦、玉米、谷子、高粱、燕麦、大麦等151个品种,以未接种材料为对照,观察并分析病毒在不同寄主上的潜育期及症状进展过程;接种30 d后,提取材料总RNA,利用一步法RT-PCR技术检测病毒侵染情况,结合生物学观察及分子生物学检测明确BYSMV对主要作物的致病性;在河北省粮食作物种植区采集580份疑似病毒病症状的植物样品,提取样品总RNA,利用一步法RT-PCR技术检测样品中BYSMV感染率,评估病害的流行风险。【结果】小麦、谷子、大麦、燕麦接种病毒9—10 d后叶片出现褪绿条纹,植株发育迟缓,严重矮化。所有供试品种均能发病,供试小麦品种发病率为60.00%—100%,谷子品种发病率为18.18%—100%,燕麦品种发病率为55.56%—100%,4个大麦品种发病率均为100%。BYSMV侵染甜(糯)玉米、高粱的潜育期为15—17 d,叶片有黄色条纹症状,植株轻微矮化;BYSMV可侵染3个甜(糯)玉米品种并表现症状,甜(糯)玉米发病率为14.29%—21.43%;BYSMV可侵染8份高粱材料,其中6个品种可表现症状,2份材料被侵染后无明显症状,发病率为4.00%—33.33%。田间调查结果显示,小麦、谷子、甜玉米样品中BYSMV检出率分别为78.03%、100%、9.52%,禾本科杂草中狗尾草、小画眉、牛筋草田间检出率分别为75.68%、65.38%、8.33%。灰飞虱接种证实,BYSMV还可侵染虎尾草、雀麦、稗草、二穗短柄草、野燕麦5种植物,发病率分别为100%、82.61%、20.00%、60.71%、10.00%,其中牛筋草、稗草、野燕麦等杂草寄主被BYSMV侵染后无明显症状。【结论】BYSMV对小麦、谷子、大麦、燕麦等禾本�【Objective】 Barley yellow striate mosaic virus(BYSMV) is widely distributed in wheat producing areas of northern China.In order to evaluate the epidemic risk of the disease and provide a theoretical foundation for formulating strategies in prevention and control,the pathogenicity to gramineous crops was determined and its natural hosts were also identified.【Method】The virus was inoculated on 151 varieties of Triticum aestivum,Zea mays,Setaria italica,Sorghum bicolor,Avena sativa,and Hordeum vulgare by planthopper(Laodelphax striatellus) carrying BYSMV,and the uninoculated materials as control.The latent period and symptom progression of the virus on different hosts were observed and analyzed.After 30 d of inoculation,one-step RT-PCR was used to detect the infection of the virus.The pathogenicity of BYSMV to main hosts was analyzed by biological and molecular biological detection.A total of 580 suspected virus-infected samples were collected from gramineous crops growing area in Hebei Province,and BYSMV was detected using one-step RT-PCR to assess the risk of BYSMV epidemic in the field.【Result】After 9-10 d of inoculation with the virus,the leaves of T.aestivum,S.italica,H.vulgare and A.sativa showed chlorotic stripes,and the plants grew slowly and were seriously dwarfed.All tested varieties of these crops were susceptible to infection.The incidence of tested T.aestivum,S.italica,A.sativa and H.vulgare varieties was 60.00%-100%,18.18%-100%,55.56%-100% and 100%,respectively.Yellow stripes and mild dwarfing symptoms could be observed after 15-17 d of virus inoculation on some Z.mays var.saccharata and S.bicolor.Among the tested Z.mays var.saccharata varieties,three varieties showed typical symptoms of BYSMV inoculation and the incidence was 14.29%-21.43%.Eight S.bicolor materials were susceptible to BYSMV with the incidence ranging from 4.00% to 33.33%,but two of them remained asymptomatic.Field surveys revealed that the positive rate of BYSMV in T.aestivum,S.italica and Z.mays var.saccharata samples was

关 键 词：大麦黄条点花叶病毒 灰飞虱 寄主 致病性 风险评估 

分 类 号：S435.123[农业科学—农业昆虫与害虫防治]