J亚型禽白血病病毒感染琅琊鸡的排毒动态及对繁殖性能的影响  

Detox Dynamics and Reproductive Performance of Langya Chickens Infected with ALV-J

作　　者：张会永[1] 武琥琮 朱国强[2] 李国辉[1] 俞燕[1] 殷建玫[1] 薛倩 周成浩 蒋一秀 苏一军[1] 黄华云[1] 韩威[1] ZHANG HuiYong;WU HuCong;ZHU GuoQiang;LI GuoHui;YU Yan;YIN JianMei;XUE Qian;ZHOU ChengHao;JIANG YiXiu;SU YiJun;HUANG HuaYun;HAN Wei(Jiangsu Institute of Poultry Science,Yangzhou 225125,Jiangsu;College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,Jiangsu;Animal Epidemic Disease Prevention and Control Center of Inner Mongolia,Hohhot 010010)

机构地区：[1]江苏省家禽科学研究所,江苏扬州225125 [2]扬州大学兽医学院,江苏扬州225009 [3]内蒙古自治区动物疫病预防控制中心,呼和浩特010010

出　　处：《中国农业科学》2024年第23期4815-4824,共10页Scientia Agricultura Sinica

基　　金：国家重点研发计划(2021YFD1200302);江苏省种业振兴揭榜挂帅(JBGS[2021]029);江苏省农业科技自主创新(CX(24)3001)。

摘　　要：【背景】我国地方鸡资源丰富,J亚型禽白血病(J subtype avian leukemia,AL-J)在地方鸡种中的传播制约着地方鸡的保种、开发利用等工作的进行。【目的】通过分析J亚型禽白血病病毒(J subtype avian leukemia virus,ALV-J)阳性琅琊鸡公鸡排毒动态、ALV-J阳性琅琊鸡母鸡在开产前至自繁时的排毒动态及ALV-J对繁殖性能(受精率、孵化率、产蛋数及高峰产蛋后产蛋下降速率)的影响,为我国地方鸡品种的AL-J净化提供参考依据,助力我国地方鸡品种的保护、开发利用。【方法】对ALV-J阴、阳性琅琊鸡母鸡群体自145日龄开始,每30 d进行血液病毒分离、泄殖腔拭子P27抗原检测;ALV-J阳性公鸡群体自330日龄开始,每隔4 d进行精液病毒分离、泄殖腔拭子P27抗原检测;进行ALV-J阴、阳性公鸡精液品质、DNA损伤程度和ALV-J阴、阳性母鸡蛋品质测定,并进行ALV-J阴、阳性公母鸡配种输精试验(G1组:阳性公鸡×阳性母鸡;G2组:阳性公鸡×阴性母鸡;G3组:阴性公鸡×阳性母鸡;G4组:阴性公鸡×阴性母鸡),统计各组种蛋的受精率和孵化率。对ALV-J阴、阳母鸡群体从开产至43周龄的周均产蛋数及高峰产蛋后产蛋下降速率进行分析。【结果】ALV-J阳性公鸡排毒动态研究表明,精液和泄殖腔拭子每次检测结果并不完全一致,部分阳性鸡仅精液或泄殖腔拭子检测为阳性,在20只阳性公鸡中有5只公鸡的精液在连续检测中呈现阴性。ALV-J阳性母鸡排毒动态结果显示,血液病毒分离检出率,205日龄最低(78.16%),325日龄最高(90.79%),265、295和325日龄呈逐步增高趋势,且295和325日龄相近。ALV-J阴、阳性鸡群血液病毒分离和泄殖腔拭子的检测结果比较说明,泄殖腔拭子检测存在较高的假阳性和漏检率。ALV-J阴、阳性公鸡常规精液品质和DNA损伤程度检测(DFI)无显著差异(P>0.05)。ALV-J阴、阳性母鸡的蛋品质测定结果显示,蛋重、哈氏单位、蛋黄重【Background】 China is rich in local chicken resources.The spread of J subtype avian leukemia(AL-J) in local chicken breeds has restricted the conservation,development and utilization of local chickens.【Objective】 By analyzing the infection dynamics of ALV-J positive Langya chickens rooters,the detox dynamics of ALV-J positive Langya chickens hens from pre-opening to self-propagation,and the effects of ALV-J on reproductive performance(fertilization rate,hatching rate,egg number and egg production decline rate after peak laying),this study provided a reference for the AL-J purification of local chickens and was helpful to the protection,development and utilization of local chicken breeds in China.【Method】 For the ALV-J negative and positive Langya chicken hens,the blood virus isolation and cloacal swab P27 antigen detection were performed every 30 days starting from the 145th day of age.Starting from the age of 330 days for the ALV-J positive rooster group,the semen virus isolation and cloacal swab P27 antigen detection were performed every 4 days.The semen quality and DNA damage degree of ALV-J negative and positive roosters were determined,as well as egg quality of ALV-J negative and positive hens.The insemination test of ALV-J negative and positive male and female chickens(G1 group:positive male chicken × positive hen;G2 group:positive male × negative hen;G3 group:negative male × positive hen;G4 group:negative male chicken × negative hen) was carried out,and the fertilization rate and hatching rate of each group of eggs were counted.The average number of eggs laid per week from the start of laying to 43weeks of age in ALV-J negative and positive hen groups as well as the egg production decline rate after peak egg production were analyzed.【Result】 Studies on the detox dynamics of ALV-J positive roosters showed that semen and cloacal swabs were not completely consistent each test,with some positive chickens testing positive only in semen or cloacal swabs,and five out of 20positive cocks testin

关 键 词：J亚型禽白血病病毒 琅琊鸡 排毒动态 繁殖性能 

分 类 号：S858.31[农业科学—临床兽医学]