不同施肥处理下烟草根际细菌网络响应  

Response of tobacco rhizosphere bacteria under different treatment of organic and microbial fertilizer

作　　者：赖先军 张文友[1] 段旺军 彭睿 相雨欣 陈梓涵 张义正 颜朗 LAI Xian-jun;ZHANG Wen-you;DUAN Wang-jun;PENG Rui;XIANG Yu-xin;CHEN Zi-han;ZHANG Yi-zheng;YAN Lang(Xichang University,Panxi Crops Reserch and Utilization Key Laboratory of Sichuan Province,Xichang,Sichuan 615013,China;China Tobacco Sichuan Industrial Co.Ltd.,Chengdu 610066,China;College of Life Sciences,Sichuan University/Sichuan Key Laboratory of Molecular Biology and Biotechnology,Chengdu 610064,China)

机构地区：[1]西昌学院/攀西特色作物研究与利用四川省重点实验室,四川西昌615013 [2]四川中烟工业有限责任公司,成都610066 [3]四川大学生命科学学院/分子生物学及生物技术四川省重点实验室,成都610064

出　　处：《西南农业学报》2024年第10期2242-2251,共10页Southwest China Journal of Agricultural Sciences

基　　金：四川中烟工业有限责任公司项目(yl2020001、SJ003-03)。

摘　　要：[目的]研究有机肥和微生物菌剂对烟草根际细菌群落结构的影响,挖掘关键响应菌群,探索烟草根际细菌对不同肥料施加的动态响应机制。[方法]聚焦烟草根际细菌对化学肥料、有机肥料和植物促生微生物(PGPMs)处理下的反应机制。通过Illumina MiSeq高通量测序技术对不同肥料处理下烟草根际细菌16S rRNA基因V3~V4区测序并对操作分类单位(OTU)进行聚类,分析处理间细菌菌群多样性特征,通过机器学习算法建立模型,将根际菌群与施肥处理相关联,同时建立细菌共现网络,解析施肥响应的关键调控菌群。[结果]与化学肥料相比,施用有机肥和微生物菌剂显著增加烟草根际细菌丰度,但降低了细菌分布的均匀性。基于模型挖掘到15个与施肥关联的指示性细菌类群,其中泛菌属(Pantoea)和寡养单胞菌属(Stenotrophomonas)细菌为微生物菌剂处理下特定菌群结构的指示性细菌。另外,从化肥、有机肥到微生物菌剂处理,根际细菌共现网络结构从松散状态转变为紧密且复杂调控状态,微生物菌剂作用下优势菌群调控增强,与周围菌群联系紧密。[结论]有机肥和微生物菌剂显著改变烟草根际细菌群落结构及根际细菌响应网络,其中泛菌属和寡养单胞菌属等微生物菌剂处理下的指示性细菌具有进一步纯化培养和商业开发价值。【Objective】The study aimed to clarify the effect of bio-organic and microbial fertilizers on tobacco rhizosphere bacterial community structure,identify key response bacterial communities,and explore the dynamic response process of tobacco rhizosphere bacteria under the treatment of bio-organic and microbial fertilizer.【Method】The study focused on the response mechanism of tobacco rhizosphere bacteria to chemical fertilizers,bio-organic fertilizers and plant growth promoting microbes(PGPMs).The Illumina MiSeq high-throughput sequencing technology was used to sequence the 16S rRNA genes V3-V4 of tobacco rhizosphere bacteria under different treatments.After clustering of operational taxonomic units(OTU),we analyzed the diversity of bacterial communities among treatments.Then we established models through machine learning methods as well as bacterial co-occurrence network to analyze the key regulatory bacteria in response to different fertilization treatments.【Result】Compared to chemical fertiliiers,the application of bio-organic and microbial fertilizer significantly increased the abundance of tobacco rhizosphere bacteria,but decreased the uniformity of the bacterial distribution.Based on the model,15 characteristic bacterial genus associated with fertilization treatment were further identified.Among them,Pantoea and Stenotrophomonas were indicator bacteria under PGPRs.The co-abundance network structure of rhizosphere bacteria changed from a loose and diverse state under chemical fertilizer treatment to a tightly and complex regulated state under microbial fertilizer treatment.Under PGPRs,the bacterial regulation enhanced and it was closely connected with the surrounding flora.【Conclusion】Bio-organic and microbial fertilizers significantly change the tobacco rhizosphere bacterial community structure and rhizosphere bacterial response network.The key regulatory bacteria,such as Pantoea and Stenotrophomonas,have the value of further purification,culture and commercial development.

关 键 词：烟草 根际细菌 细菌多样性 指示性细菌 细菌共现网络 

分 类 号：S154.3[农业科学—土壤学]