川西高原大棚感病番茄根际土壤细菌群落特征  

Characteristics of bacterial communities in rhizosphere soil of greenhouse susceptible tomatoes in the western Sichuan Plateau

作　　者：王谢[1,2] 李志 李芹[1] 阳路芳 吴翔 常伟[4,5,6] WANG Xie;LI Zhi;LI Qin;YANG Lu-fang;WU Xiang;CHANG Wei(Institute of Agricultural Resources and Environment,Sichuan Academy of Agricultural Sciences,Chengdu 610066,China;Key Laboratory for Southwest Mountain Agriculture Environment,Ministry of Agriculture and Rural Affairs,Chengdu 610066,China;Horticulture Research Institute,Sichuan Academy of Agricultural Sciences,Chengdu 610066,China;Key Laboratory for Vegetable Germplasm and Variety Innovation in Sichuan Province,Chengdu 610066,China;Key Laboratory for Horticultural Crop Biology and Germplasm Creation in Southwest China,Ministry of Agriculture and Rural Affairs,Chengdu 610066,China;Sichuan Institute of Edible Fungi,Chengdu 610066,China)

机构地区：[1]四川省农业科学院农业资源与环境研究所,成都610066 [2]农业农村部西南山地农业环境重点实验室,成都610066 [3]四川省农业科学院园艺研究所,成都610066 [4]蔬菜种质与品种创新四川省重点实验室,成都610066 [5]农业农村部西南地区园艺作物生物学与种质创制重点实验室,成都610066 [6]四川省食用菌研究所,成都610066

出　　处：《西南农业学报》2024年第10期2261-2269,共9页Southwest China Journal of Agricultural Sciences

基　　金：国家重点研发计划项目(2022YFD1601503);四川省科技计划项目(2021YFYZ0022);四川省农科院中试熟化项目(2024ZS SFXD06)。

摘　　要：[目的]探索川西高原大棚番茄感病后根际细菌群落的响应状况。[方法]利用高通量技术分析四川省甘孜州道孚县和炉霍县高原大棚健康番茄和发病番茄根际土壤微生物群落的结构特征。[结果](1)番茄发病状况可解释土壤细菌群落结构23%的总方差。(2)道孚县土壤样品的细菌物种数比炉霍县略高,发病植株比健康植株的细菌物种数略低,但都未达到显著水平。(3)暖发菌门、厚壁菌门、候选门GAL15、螺旋体门的相对丰度在番茄发病后明显降低,而绿弯菌门、候选门RCP2-54、候选门SAR324、异常球菌门的相对丰度则在番茄发病后明显增加。(4)土壤细菌群落结构的7类功能参与了川西高原大棚番茄的健康管理,其中道孚县土壤细菌的铁呼吸功能减弱、芳香化合物降解功能增强,而炉霍县的发酵功能得到较大提升。(5)番茄根际土壤中,道孚县有127个菌种显著响应发病,炉霍县有64个菌种显著响应发病。(6)道孚县根际细菌结构与功能变化源于土壤pH的降低,而炉霍县根际细菌结构与功能变化源于土壤有机质含量的增加。[结论]川西高原大棚土壤微生物功能健全,且在番茄发病过程中能够主动招募有益微生物菌群以应对病害,如道孚县的芽单胞菌属(Gemmatimonas sp.)和炉霍县的异常球菌门细菌(Deinococcota)。【Objective】The present study aimed to explore the response of rhizosphere bacterial communities in greenhouse tomatoes infected with diseases in the western Sichuan Plateau.【Method】The high-throughput technology was used to analyze the structural characteristics of rhizosphere soil microbial communities of diseased and healthy tomatoes in plateau greenhouses in Daofu county and Luhuo county,Ganzi prefecture,Sichuan province.【Result】(i)The explanatory rate of disease incidence on the total variance of soil bacterial community structure in all samples was 23%.(ii)The number of bacterial species in soil samples from Daofu county was slightly higher than that of Luhuo county,while the number of bacterial species in diseased plants was slightly lower than that in healthy plants,but none of them had reached a significant level.(iii)The relative abundance of Warms,Firmicutes,Candidate GAL15 and Spirochetes decreased significantly after tomato disease onset,while the relative abundance of Chlorobacteria,Candidate RCP2-54,Candidate SAR324 and Abnormalities increased significantly after tomato disease onset.(iv)The seven functions of soil bacterial community structure were involved in the health management of greenhouse tomatoes in the western Sichuan Plateau.Among them,the iron respiration function of soil bacteria in Daofu county was weakened,the degradation function of aromatic compounds was enhanced,and the fermentation function in Luhuo county had been greatly improved.(v)In the rhizosphere soil of tomatoes,127 bacterial strains significantly responded to the disease in Daofu county and 64 bacterial strains significantly responded to the disease in Luhuo county.The changes in the structure and function of rhizosphere bacteria in Daofu county were due to the decrease in soil pH,while the changes in the structure and function of rhizosphere bacteria in Luhuo county were due to the increase in organic carbon.【Conclusion】The results reflects a sound microbial function of greenhouse soil in the western Sich

关 键 词：高原 番茄 根际 微生物 多样性 

分 类 号：S154.1[农业科学—土壤学]