丝胶蛋白对小鼠DC2.4细胞成熟及抗原提呈功能的影响  

Effects of Sericin on Maturity and Antigen-Presenting Function of DC2.4 Cell from Mouse

作　　者：李海波 李海文 张兆林 骆发增 Li Haibo;Li Haiwen;Zhang zhaolin;Luo Fazeng(Foshan Institute of Pathogenic Microbiology,Foshan Guangdong 528000,China;Maternal and Child Health Hospital of Yuexiu District of Guangzhou,Guangzhou 510000,China;Zhongzhi(Shandong)Biotechnology Co.,Ltd,Heze Shandong 274000,China)

机构地区：[1]佛山病原微生物研究院,广东佛山528000 [2]广州市越秀区妇幼保健院,广州510000 [3]中植(山东)生物技术有限责任公司,山东菏泽274000

出　　处：《蚕业科学》2024年第5期433-439,共7页ACTA SERICOLOGICA SINICA

摘　　要：抗原提呈在免疫反应中发挥重要作用,是激活特异免疫系统的唯一机制。丝胶蛋白已被证实具有提高天然性免疫的作用。为探讨丝胶蛋白对小鼠树突状细胞抗原提呈功能的影响,进一步阐明丝胶蛋白增强免疫反应的分子机制,通过体外实验研究丝胶蛋白对DC2.4细胞增殖、成熟度和表面分子表达的影响,以及通过体内实验研究丝胶蛋白对小鼠抗体水平的影响。流式细胞仪检测结果显示,丝胶蛋白能明显提高成熟DC2.4细胞表面分子MHCⅡ和CD86的表达水平。CCK8法检测发现丝胶蛋白能促进DC2.4细胞增殖,最佳丝胶蛋白浓度为1 mg/mL以内;且可显著促进DC2.4刺激的T淋巴细胞增殖。用模式抗原OVA免疫小鼠,ELISA法检测发现丝胶蛋白处理组小鼠血清中的IgG和IgG1抗体含量分别比PBS空白组高30%~40%。综上,丝胶蛋白可能通过促进DC2.4细胞成熟,增强DC2.4细胞的抗原呈递作用并进一步刺激T细胞增殖,促进特异性免疫应答。研究结果为丝胶蛋白的现代免疫学应用提供了参考。Antigen presentation is the only mechanism to activate specific immune system,playing the important role in immune response.Sericin has been proved to have the important effect of improving the natural immunity.In order to explore the effect of sericin on the antigen presentation function of dendritic cell in mice,and further elucidate the molecular mechanism of sericin enhancing immune response,this article investigated the effects of sericin on the proliferation,maturity,and surface molecule expression of DC2.4 cell through in vitro experiments,as well as the effects of sericin on antibody levels in mice through in vivo experiments.Flow cytometry results showed that sericin could significantly enhance the expression levels of surface molecules MHCⅡand CD86 in mature DC2.4 cell.CCK8 method showed that sericin promoted the proliferation of DC2.4 cell,and the optimal concentration of sericin was within 1 mg/mL.The sericin treatment group had obvious proliferation effect on T cell stimulated by DC2.4.The mice were immunized with model antigen OVA,and ELISA detection showed that the contents of antibody IgG and IgG1 in serum of mice in sericin treatment group was 30%-40%higher than those in PBS treatment group.To sum up,sericin could promote specific immune response by promoting the maturation of DC2.4 cell,enhancing the antigen presentation of DC2.4 cell and accelerating the proliferation of T lymphocyte.These above results can provide reference for evaluating modern immunological applications of sericin.

关 键 词：丝胶蛋白 DC2.4细胞 抗原提呈 

分 类 号：S886.9[农业科学—特种经济动物饲养]