1株西藏来源链霉菌10-37的次级代谢产物研究  

Secondary metabolites of Streptomyces sp.10-37 from Xizang

作　　者：王嘉涵 常珊珊[2] 陈铭旭 何宁[2] 王梦源 解云英[2] 袁丽杰 Wang Jiahan;Chang Shanshan;Chen Mingxu;He Ning;Wang Mengyuan;Xie Yunying;and Yuan Lijie(Tangshan Key Laboratory for Preclinical and Basic Research on Chronic Disease,Hebei Key Laboratory for Chronic Diseases,School of Basic Medical Science,North China University of Science and Technology,Tangshan 063210;CAMS Key Laboratory of Synthetic Biology for Drug Innovation,Institute of Medicinal Biotechnology,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100050)

机构地区：[1]华北理工大学基础医学院,河北省慢性疾病基础医学重点实验室,唐山市慢性病临床基础研究重点实验室,唐山063210 [2]中国医学科学院&北京协和医学院,医药生物技术研究所,药物合成生物学重点实验室,北京100050

出　　处：《中国抗生素杂志》2024年第11期1289-1296,共8页Chinese Journal of Antibiotics

基　　金：国家自然基金面上项目(No.81973219);国家自然基金青年项目(No.82104047);中国医学科学院医学与健康科技创新工程项目(No.2021-I2M-1-028);河北省自然科学基金(No.H2021209027)。

摘　　要：目的对1株西藏来源链霉菌10-37的次级代谢产物进行研究。方法在分子网络指导下,利用HW40 C凝胶柱、Flash快速液相分离制备色谱仪、半制备型高效液相色谱等方法对化合物进行分离纯化,运用核磁共振、高分辨液质联用技术对单体化合物进行结构鉴定,最后利用微量肉汤稀释法进行抗菌活性测定。结果从链霉菌10-37的菌丝体提取物中分离得到了4个代谢产物,包括2个新化合物oxazolomycin A3(1)和A4(2),以及2个已知化合物oxazolomycin A2(3)和oxazolomycin B(4)。其中化合物1和2为化合物3的一对几何异构体,它们三烯部分的构型分别为(4’E,6'E,8'E)(1)、(4’Z,6'E,8'E)(2)、(4’Z,6'Z,8'E)(3)、(4’E,6'E,8'E)(4)。结论西藏特境的地理优势结合分子网络分析的策略,可以更高效地挖掘新结构次级代谢产物。Objective This research studied the secondary metabolites of Streptomyces sp.10-37 collected in Xizang.Methods Under the guidance of the molecular network,the compounds were separated and purified by HW40 C gel columns,flash rapid liquid separation and preparation chromatographs,semi-preparative highperformance liquid chromatography and other methods.The structures of the purified compounds were identified by nuclear magnetic resonance and high-resolution liquid chromatography-mass spectrometry.Finally,the antibacterial activity was determined by the microbroth dilution method.Results Four metabolites were isolated and identified from the mycelium extract of Streptomyces sp.10-37,including two new compounds,oxazomycin A3(1)and A4(2),as well as two known compounds,oxazomycin A2(3)and oxazomycin B(4).Compounds 1 and 2 were a pair of geometric isomers of 3,with the configurations of their diene moieties being(4'E,6'E,8'E)(1),(4'Z,6'E,8'E)(2),(4'Z,6'Z,8'E)(3),and(4'E,6'E,8'E)(4),respectively.Conclusion The geographical advantages of Xizang's special environment,combined with the strategy of molecular network analysis,can better excavate new secondary metabolites.

关 键 词：链霉菌 次级代谢产物 结构鉴定 分子网络 

分 类 号：R978.1[医药卫生—药品]