233份中果番茄分离单株的抗病基因检测  

Detection of Disease Resistance Genes in 233 Medium Fruit Tomato Isolates

作　　者：王珊珊 王晨瑜 杨超沙 蔡晓艺 尹庆珍[1] WANG Shanshan;WANG Chenyu;YANG Chaosha;CAI Xiaoyi;YIN Qingzhen(Institute of Cash Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050051;Chengde Academy of Agriculture and Forestry Sciences,Chengde 067055)

机构地区：[1]河北省农林科学院经济作物研究所,石家庄050051 [2]承德市农林科学院,河北承德067055

出　　处：《中国农学通报》2024年第34期24-32,共9页Chinese Agricultural Science Bulletin

基　　金：河北省重点研发计划项目“果菜类蔬菜现代种业科技创新团队——优质多抗设施番茄种质资源创制与新品种选育”(21326309D);河北省重点研发计划项目“高品质、多抗樱桃番茄种质资源创制与新品种选育”(22326313D);河北省农林科学院——科技创新专项课题“设施番茄新品种选育及棚室装备研究应用”(2022KJCXZX-JZS-5);河北省农林科学院科技创新人才队伍建设——博士基金“番茄高糖基因Lin5的发掘与利用研究”(C22R0801);河北省农林科学院基本科研业务费“番茄苹果酸相关基因的定位及育种利用”(2024050203);河北省第三批“巨人计划”——蔬菜科研创新团队项目。

摘　　要：通过筛选多抗中果番茄资源,为番茄抗病育种提供材料基础。以课题组引进的28个中果番茄品种为材料,每个品种选择8~10株分离单株的幼嫩叶片,采用五引物扩增受阻突变体系(PARMS)法对其233份分离单株进行12种病害的17个抗性基因检测,分别为黄化曲叶病毒病抗性基因Ty-1、Ty-5,烟草花叶病毒抗病基因Tm-2a,根结线虫抗性基因Mi1.2、Mi2.3,根腐病抗性基因Frl,叶霉病抗性基因Cf-5、Cf-9,灰叶斑病抗性基因Sm,枯萎病抗性基因I-2,晚疫病抗性基因Ph-3,青枯病抗性基因Bwr12、Bwr6,细菌性斑点病抗性基因Pto,疮痂病抗性基因Rx4,黄萎病抗性基因Ve1、Ve2。结果显示,在233份分离单株中含有抗性基因Ve1、Sm、Ve2、Mi1.2的分离单株较多,分别为222、215、198、194份。经统计含有7、8个抗性基因的分离单株较多,分别为55、50份;含8个及以上抗性基因的共140份,占比60%。综合抗性最终筛选到3个含有12个抗性基因的分离单株,分别为2-2、5-4、21-3;含有11个抗性基因的有21个,分别为1-1、1-3、2-3、2-4、2-7、2-8、4-2、4-4、4-5、5-7、5-8、8-1、8-7、8-8、12-1、12-2、12-5、18-7、18-8、20-8、26-2,多抗资源较为丰富。本研究获得了多个多抗中果番茄资源,下一步可对筛选的分离单株进行纯化,为筛选多抗中果番茄提供基础材料。In order to screen the resources of medium fruit tomato with multiple resistances and provide material basis for tomato disease resistance breeding,using 28 medium fruit tomato varieties introduced by our research group as materials,17 resistance genes of 12 diseases were detected by five-primer amplification inhibited mutation system(PARMS)method,including yellow leaf curl virus resistance genes Ty-1 and Ty-5,tobacco mosaic virus resistance gene Tm-2a,root nematode resistance genes Mi1.2 and Mi2.3,Fusarium crown and root rot resistance gene Frl,leaf mold resistance genes Cf-5 and Cf-9,grey leaf spot resistance genes Sm,wilt resistance gene I-2,late blight resistance gene Ph-3,bacterial wilt resistance genes Bwr12 and Bwr6,bacterial speck resistance gene Pto,bacterial spot resistance gene Rx4,Verticillium wilt resistance gene Ve1 and Ve2.Among the 233 isolates,there were 222,215,198 and 194 isolates containing the resistance genes Ve1,Sm,Ve2 and Mi1.2,respectively.The isolates with 7 and 8 resistance genes were 55 and 50,respectively.140 isolates were with 8 or more resistance genes,accounting for 60%.3 isolated strains containing 12 resistance genes were screened,which were 2-2,5-4 and 21-3.And there were 21 isolated strains containing 11 resistance genes,which were 1-1,1-3,2-3,2-4,2-7,2-8,4-2,4-4,4-5,5-7,5-8,8-1,8-7,8-8,12-1,12-2,12-5,18-7,18-8,20-8,26-2.Polyresistant tomato resources were abundant.In this study,a number of medium fruit tomato resources with multiple antibodies were obtained,and the next step can be purification of the isolated single strain,providing a material basis for screening medium fruit tomato with multiple antibodies.

关 键 词：中果番茄 分离单株 五引物扩增受阻突变体系 抗性检测 

分 类 号：S641.2[农业科学—蔬菜学]