犬卵巢颗粒细胞瘤的诊断和治疗  

Diagnosis and Treatment of Canine Ovarian Granulosa Cell Tumor

作　　者：叶蒂 王鹿敏 李琦 张华[1,2] 师福山 林琳[4] 王华南 YE Di;WANG Lumin;LI Qi;ZHANG Hua;SHI Fushan;LIN Lin;WANG Huanan(Zhejiang University Center for Veterinary Sciences,Hangzhou 310058,China;College of Animal Sciences,Zhejiang University,Hangzhou 310058,China;Hangzhou Sanqian Pet Hospital Co.,Ltd.,Hangzhou 310050,China;MetaHealth Veterinary Diagnostic Laboratory,Hangzhou 310059,China)

机构地区：[1]浙江大学动物医学中心,浙江杭州310058 [2]浙江大学动物科学学院,浙江杭州310058 [3]杭州三千宠物医院有限公司,浙江杭州310050 [4]元医动物医学诊断实验室,浙江杭州310059

出　　处：《中国兽医杂志》2025年第1期14-18,共5页Chinese Journal of Veterinary Medicine

基　　金：中央高校基本科研业务费专项资金(2022-KYY-517101-0005);杭州城西科创大走廊创新发展专项资助(B231220.0005-46)。

摘　　要：犬卵巢颗粒细胞瘤在我国较为罕见。本院接诊了1例因咳嗽、呼吸急促转诊的患犬病例。经实验室检查和影像学检查确诊为卵巢肿瘤并伴有右肺中叶实变、胸腔积液和腹腔积液。肿瘤经手术切除后进一步做组织病理学检查。苏木精-伊红(H.E.)染色结果显示,卵巢组织结构消失,肿瘤细胞形态多样,呈实性片状、索状、小梁状或巢状排列,肿瘤细胞胞质丰富,细胞核呈空泡状,核分裂罕见,可见核仁,染色质粗大;免疫组织化学染色结果显示,卵巢颗粒细胞瘤特异性标记物抑制素-α(INH-α)表达5%阳性,非特异性标记物肾母细胞瘤蛋白1(WT1)和白细胞分化抗原10(CD10)表达阳性。患犬最终确诊为卵巢颗粒细胞瘤,临床分期判定为恶性。该犬在切除肿瘤后第3年复查,状态良好,未见复发,但仍需持续观察。本文旨在对犬卵巢颗粒细胞瘤的诊疗流程进行总结,以供临床参考。Ovarian granulosa cell tumors in dogs are relatively rare in China.This report presents a case of a dog referred for cough and dyspnea.Laboratory and imaging examinations confirmed an ovarian tumor associated with right middle lobe consolidation,pleural effusion,and peritoneal effusion.The tumor was surgically removed,and further histopathological examination was performed.Hematoxylin-eosin(H.E.)staining revealed loss of ovarian tissue architecture,with tumor cells showing a variety of morphologies,including solid sheets,cords,trabecular,or nest-like arrangements.The cytoplasm was abundant,and the nuclei appeared vacuolated,with rare mitosis.Nucleoli were visible,and chromatin was coarse.Immunohistochemical staining results showed that the specific marker inhibin-α(INH-α)was 5%positive for ovarian granulosa cell tumors,while non-specific markers wilms tumor 1(WT1)and cluster of differentiation 10(CD10)were positive.The dog was diagnosed with an ovarian granulosa cell tumor and staged as malignant.The dog had a good recovery after surgery and was in stable condition at the 3-year follow-up with no recurrence,though continued observation was recommended.This report aims to summarize the diagnostic and treatment processes for ovarian granulosa cell tumors in dogs,providing valuable reference for clinical practice.

关 键 词：犬 腹腔肿瘤 卵巢颗粒细胞瘤 免疫组织化学 

分 类 号：S857.3[农业科学—临床兽医学]