卵黄蛋白原2对地塞米松诱导肥胖的治疗作用  

Therapeutic Effect of Vitellogenin 2 on Dexamethasone-Induced Obesity

作　　者：张慧玲 姚志超 孙雅琴 李艺雷 罗小毛 王海东[1] ZHANG Huiling;YAO Zhichao;SUN Yaqin;LI Yilei;LUO Xiaomao;WANG Haidong(College of Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China;College of Veterinary Medicine,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]山西农业大学动物医学学院,山西太谷030801 [2]河南农业大学动物医学院,河南郑州450046

出　　处：《中国兽医杂志》2025年第1期55-64,共10页Chinese Journal of Veterinary Medicine

基　　金：山西省“1331工程”(20211331-16);山西省基础研究计划(202303021211092);山西农业大学“科技创新提升工程”(CXGC2023017)。

摘　　要：卵黄蛋白原2(VTG2)被证明可促进3T3-L1脂肪细胞棕色化进程,但其是否能通过促进体内脂肪组织棕色化以治疗肥胖的作用尚未见报道。本试验为探索VTG2对小鼠白色脂肪组织(WAT)棕色化的影响,设置腺相关病毒空载组(AAV9-NC1)和VTG2处理组(AAV9-VTG2),利用苏木精-伊红(H.E.)染色观察WAT脂肪细胞面积大小,免疫组织化学(IHC)染色法、实时荧光定量PCR(qPCR)和蛋白免疫印迹(WB)法检测WAT棕色化标志因子解偶联蛋白-1(UCP1)和过氧化物酶体增殖物激活受体γ共激活剂1-alpha(PGC1α)的表达;为进一步探究VTG2对地塞米松(DEX)诱导的肥胖小鼠中WAT棕色化的影响,设置腺相关病毒空载组(AAV9-NC2组)、DEX处理组(AAV9-NC+DEX组)和VTG2治疗组(AAV9-VTG2+DEX组),采用上述方法检测相同指标。结果显示,与AAV9-NC1组相比,AAV9-VTG2组小鼠腹股沟WAT脂肪细胞面积极显著减小(P<0.01),UCP1和PGC1α的mRNA相对表达量均极显著升高(P<0.001或P<0.01),蛋白相对表达量均显著上调(P<0.01或P<0.05);与AAV9-NC2组相比,AAV9-NC+DEX组小鼠腹股沟WAT脂肪细胞面积极显著增加(P<0.01),UCP1和PGC1α的mRNA相对表达量均极显著降低(P<0.001或P<0.01),蛋白相对表达量均极其显著下调(P<0.001);与AAV9-NC+DEX组相比,AAV9-VTG2+DEX组中小鼠腹股沟WAT脂肪细胞面积极其显著减小(P<0.001),UCP1和PGC1α的mRNA相对表达量均极显著升高(P<0.001或P<0.01),蛋白相对表达量均显著上调(P<0.05)。综上表明,VTG2可促进WAT棕色化,对DEX诱导的肥胖具有抵抗作用,为治疗肥胖提供新的研究思路。Vitellogenin 2(VTG2)has been shown to promote the browning process of 3T3-L1 adipocytes,but its potential role in treating obesity by promoting the browning of adipose tissue in vivo has not been reported.This study aims to explore the effects of VTG2 on the browning of white adipose tissue(WAT)in mice.The study involved two groups:the adeno-associated virus(AAV)empty vector group(AAV9-NC1)and the VTG2 treatment group(AAV9-VTG2).Hematoxylin-eosin(H.E.)staining was used to observe the size of WAT adipocytes,while immunohistochemistry(IHC),real-time quantitative polymerase chain reaction(qPCR),and Western blotting(WB)were used to detect the expression of the browning markers uncoupling protein-1(UCP1)and peroxisome proliferator-activated receptor gamma coactivator 1-alpha(PGC1α)in WAT.To further investigate the effect of VTG2 on dexamethasone(DEX)-induced browning of WAT in obese mice,three groups were established:the AAV empty vector group(AAV9-NC2),the DEX treatment group(AAV9-NC+DEX),and the VTG2 treatment group(AAV9-VTG2+DEX).The same methods were applied to detect the same markers.The results showed that,compared with the AAV9-NC1 group,the AAV9-VTG2 group had significantly smaller inguinal WAT adipocytes(P<0.01),and the mRNA expression levels of UCP1 and PGC1αwere significantly higher(P<0.001 or P<0.01),with protein levels also significantly upregulated(P<0.01 or P<0.05).Compared with the AAV9-NC2 group,the AAV9-NC+DEX group had significantly larger inguinal WAT adipocytes(P<0.01),with a marked decrease in the mRNA expression of UCP1 and PGC1α(P<0.001 or P<0.01),and the protein levels were significantly reduced(P<0.001).Furthermore,compared with the AAV9-NC+DEX group,the AAV9-VTG2+DEX group had significantly smaller inguinal WAT adipocytes(P<0.001),and the mRNA expression levels of UCP1 and PGC1αwere significantly higher(P<0.001 or P<0.01),with protein levels significantly upregulated(P<0.05).In conclusion,VTG2 promotes the browning of WAT and has a protective effect against DEX-induced obesity,providing

关 键 词：卵黄蛋白原2(VTG2) 腹股沟 白色脂肪组织(WAT) 地塞米松 棕色化 

分 类 号：S852.21[农业科学—基础兽医学]