牛结核病大罐奶抗体检测方法的评估  

Evaluation of Antibody Detection Method for Bovine Tuberculosis in Bulk Tank Milk

作　　者：陈颖钰[1,2,3,4] 张琼文 王杰[1,2,3] 陈洁 郭爱珍 CHEN Yingyu;ZHANG Qiongwen;WANG Jie;CHEN Jie;GUO Aizhen(National Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University,Wuhan 430070,China;College of Veterinary Medicine,College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology,Huazhong Agricultural University,Wuhan 430070,China;National Professional Laboratory for Animal Tuberculosis(Wuhan),Huazhong Agricultural University,Wuhan 430070,China;Wuhan Institute of Animal Science and Veterinary Medicine,Wuhan 430065,China)

机构地区：[1]华中农业大学农业微生物资源发掘与利用全国重点实验室,湖北武汉430070 [2]华中农业大学动物科技学院动物医学院,湖北武汉430070 [3]华中农业大学湖北省兽医流行病学国际科技合作基地,湖北武汉430070 [4]华中农业大学国家动物结核病专业实验室(武汉),湖北武汉430070 [5]武汉市畜牧兽医科学研究所,湖北武汉430065

出　　处：《中国兽医杂志》2025年第1期75-79,共5页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金联合基金项目(U21A20259);湖北省自然科学基金动物源性人兽共患传染病防控研究创新群体(2021CFA016);国家现代农业(肉牛/牦牛)产业技术体系(CARS-37)。

摘　　要：为了评估大罐奶抗体检测是否可用于牛结核病的监测和预警,本试验利用酶联免疫吸附试验(ELISA)抗体检测法,对采自湖北省3家规模化奶牛场的85头奶牛的奶样与其对应的血样进行牛结核病抗体相关性分析;并通过检测27家奶牛场的大罐奶和2998份个体奶样,分析大罐奶抗体水平与牛结核病流行率之间的相关性,建立基于大罐奶抗体检测的牛结核病的流行率预测模型;为确定不同效价阳性奶样在大罐奶中的检测能力,通过对不用阳性奶样进行稀释后混样检测,分析阳性奶样的效价和具有相近大罐奶S/P值的牛群中动物个体的抗体水平经验分布函数的差异性。结果显示,奶样和血样的牛结核病抗体检测结果之间具有高度一致性(Kappa=0.862)和相关性(Pearson相关系数r=0.976,P<0.01)。大罐奶抗体水平检测结果与牛结核病流行率之间具有中度相关性(Pearson相关系数r=0.681,P<0.01),流行率预测模型y=e4.7010+1.8784ln(x)-0.01(R2=0.8933)的预测能力为89.33%。分别稀释不同效价的阳性奶样,牛结核弱阳性奶样2倍稀释后阳性检出率为16.67%,中阳性奶样16倍稀释后阳性检出率为50.00%,高阳性奶样16倍稀释后阳性检出率为100%。结果表明,奶样可用于牛结核病的抗体检测,且大罐奶抗体水平与牛结核病流行率之间具有相关性,并可以通过模型预测牛结核病的流行率。大罐奶抗体检测可作为一项经济快捷的检测手段用于牛结核病的监测和预警,对于有着较高流行率或存在有强阳性个体的牛场,该检测方法敏感性更高,更为适用。In order to assess whether the detection of antibodies in bulk tank milk can be used for monitoring and early warning of bovine tuberculosis,this study utilized enzyme-linked immunosorbent assay(ELISA)antibody detection method to analyze the correlation of bovine tuberculosis antibodies between milk samples and their corresponding blood samples from 85 dairy cows in three large-scale dairy farms in Hubei Province.By detecting bulk tank milk from 27 dairy farms and 2998 individual milk samples,the correlation between the antibody levels in bulk tank milk and the prevalence of bovine tuberculosis was analyzed.A predictive model for bovine tuberculosis prevalence based on bulk tank milk antibody detection was established.To determine the detection capability of different potency positive milk samples in bulk tank milk,positive milk samples were diluted and mixed for detection,and differences in the empirical distribution functions of antibody levels among individual animals with similar bulk tank milk S/P values were analyzed.The results showed a high consistency(Kappa=0.862)and correlation(Pearson correlation coefficient r=0.976,P<0.01)between bovine tuberculosis antibody detection results in milk samples and blood samples.There was a moderate correlation between bulk tank milk antibody levels and the prevalence of bovine tuberculosis(Pearson correlation coefficient r=0.681,P<0.01).The predictive ability of the prevalence model y=e4.7010+1.8784ln(x)-0.01(R2=0.8933)was 89.33%.When diluting positive milk samples of different titers,the positive detection rates after 2-fold dilution of weak-positive milk samples,16-fold dilution of moderate-positive milk samples,and 16-fold dilution of strong-positive milk samples were 16.67%,50.00%,and 100%,respectively.The results indicate that milk samples can be used for bovine tuberculosis antibody detection,and there is a correlation between bulk tank milk antibody levels and the prevalence of bovine tuberculosis.The prevalence of bovine tuberculosis can be predicted through the

关 键 词：牛结核病 大罐奶 效价 预测模型 

分 类 号：S855.2[农业科学—临床兽医学]