我国猫星状病毒流行情况调查及其分子特性分析  

Epidemiological Investigation and Molecular Characterization of Feline Astrovirus in China

作　　者：杜雯欣 李勇璠 曾悦 颜权辉 李秋燕 曹胜波[1,2] 周登元 李佳康 Du Wenxin;Li Yongfan;Zeng Yue;Yan Quanhui;Li Qiuyan;Cao Shengbo;Zhou Dengyuan;Li Jiakang(College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,Hubei,China;National Key Laboratory of Agricultural Microbial Resources Exploitation and Utilization,Huazhong Agricultural University,Wuhan 430070,Hubei,China;Wuhan Keqian Biology Co.,Ltd,Wuhan 430040,Hubei,China)

机构地区：[1]华中农业大学动物医学院,湖北武汉430070 [2]华中农业大学农业微生物资源发掘与利用全国重点实验室,湖北武汉430070 [3]武汉科前生物股份有限公司,湖北武汉430040

出　　处：《中国动物检疫》2024年第12期8-15,共8页China Animal Health Inspection

基　　金：国家重点研发计划项目(2023YFD1800402);湖北省试验动物研究项目(2023CFA005)。

摘　　要：为了解我国猫星状病毒(FeAstV)的流行趋势及遗传进化特点,2023年在我国7大行政片区的16个省级行政区采集了2560份猫临床样品,通过RT-PCR方法对样品进行了FeAstV检测并对主要免疫原性蛋白VP90进行了遗传进化分析。结果显示:FeAstV总检出率为8.01%(205/2560),有6个行政片区存在FeAstV感染(华东片区除外);未成年猫、母猫、群居猫的FeAstV检出率相对较高;测序阳性样品与参考毒株的氨基酸同源性为68.7%~97.7%,分为A、B2个基因群,不同群间VP90蛋白氨基酸同源性较低(68.6%~73.5%);2个基因群FeAstVVP90蛋白的第590~700位氨基酸区域均具有高突变性。结果说明:FeAstV在我国流行范围较广,猫年龄、性别及其饲养环境对FeAstV传播有显著影响,FeAstV持续发生变异且具有一定人兽共感染隐患,需要对其加强监测。In order to identify the prevalence trend and genetic evolution characteristics of feline asterovirus(FeAstV)in China,2560 samples were collected from cats in 16 administrative divisions at the province level in 7 administrative regions in China in 2023,and detected for FeAstV using RT-PCR,followed by genetic evolution analysis on the major immunogenic protein VP90.The results showed that the total detection rate of FeAstV was 8.01%(205/2560),and FeAstV infection was present in 6 administrative regions(excluding East China);FeAstV was mostly detected from juvenile cats,female cats and gregarious cats;the amino acid homology between the positive samples and the reference strains was from 68.7%to 97.7%,and the detected strains could be divided into two genetic groups,A and B,and the amino acid homology of VP90 protein among different groups was relatively low(from 68.6%to 73.5%);high mutability was found in the 590-700 amino acid regions of FeAstV VP90 protein in the two groups.In conclusion,FeAstV was widely prevalent in China with continuous variation,and its transmission was influenced by the age,sex and feeding environment of cats,posing a potential risk of zoonotic infection,which should be intensively monitored.

关 键 词：猫星状病毒 ORF2基因 VP90蛋白 遗传进化分析 

分 类 号：S855.3[农业科学—临床兽医学]