河南省腹泻病死仔猪主要病毒性腹泻病原调查  

Investigation on the Major Viral Pathogens in Piglets Dead of Diarrhea in Henan Province

作　　者：房大学[1] 刘光辉[1] 王华俊[1] 赵胜杰[1] 赵美雪 宋丹 郭洛生[1] Fang Daxue;Liu Guanghui;Wang Huajun;Zhao Shengjie;Zhao Meixue;Song Dan;Guo Luosheng(Henan Center for Animal Disease Prevention and Control,Henan Key Laboratory of Animal Disease Monitoring,Early Warning,Prevention and Control,Zhengzhou 450008,Henan,China)

机构地区：[1]河南省动物疫病预防控制中心,河南省重大动物疫病监测预警及防控重点实验室,河南郑州450008

出　　处：《中国动物检疫》2024年第12期44-48,82,共6页China Animal Health Inspection

基　　金：河南省重点研发专项(231111111300)。

摘　　要：为了解导致河南省仔猪病毒性腹泻的主要病原种类及分布特征,2022年在豫东、豫南、豫西、豫北、豫中等地区无害化处理厂采集临床腹泻病死仔猪的肠管及其内容物样品共计496份,采用三重荧光RT-PCR检测猪传染性胃肠炎病毒(TGEV)、猪流行性腹泻病毒(PEDV)、猪轮状病毒(PoRV)、猪冠状病毒(PDCoV)及猪急性腹泻综合征冠状病毒(SADS-CoV),并分析不同季节、不同地区以及不同病毒混合感染情况。检测结果显示:496份腹泻病料样品中,PoRV、PDCoV阳性检出率(7.66%、8.27%)相对较高,SADS-CoV未被检出;不同季节统计结果显示,TGEV仅在春季检出,PEDV、PoRV在春、冬两季检出,PDCoV一年四季均有检出;不同区域检测结果显示,TGEV仅在豫东被检出,PEDV在豫北的阳性检出率最高,PoRV、PDCoV在豫西的阳性检出率(16.67%、26.67%)最高;病毒混合感染统计结果显示,以2种病毒混合感染为主,其中PEDV+PDCoV阳性检出率最高(1.81%)。结果表明,河南省仔猪病毒性腹泻主要发生在冬、春季节,PEDV、PoRV、PDCoV为主要病毒性病原,且存在混合感染情况,其感染存在一定的区域特征。本研究结果为河南省养猪场和畜牧兽医主管部门制定猪病毒性腹泻防控策略提供了数据支持。In order to identify the major viral pathogens leading to diarrhea in piglets in Henan Province and their distribution characteristics,a total of 496 samples of intestinal tracts and their contents were collected from piglets dead of diarrhea in biosafety disposal plants in the Eastern,Southern,Western,Northern and Central Henan Province in 2022 to detect transmissible gastroenteritis virus(TGEV),porcine epidemic diarrhea virus(PEDV),porcine rotavirus(PoRV),porcine delta coronavirus(PDCoV)and swine acute diarrhea syndrome coronavirus(SADSCoV)using triple fluorescence RT-PCR,and the infection in different seasons and regions as well as mixed infection of different viruses was analyzed.The results showed that the positive detection rates of PoRV and PDCoV(7.66%and 8.27%)were relatively high,and no SADS-CoV was detected;the statistical results based on different seasons showed that TGEV was detected only in springs,PEDV and PoRV were in springs and winters,and PDCoV was in all seasons throughout the year;the test results based on different regions showed that TGEV was detected only in the eastern Henan,PEDV was mainly in the northern Henan,PoRV and PDCoV were mainly in the western Henan(16.67%and 26.67%,respectively);and the statistical results based on viral mixed infection showed that mixed infection with 2 viruses were predominant,especially PEDV+PDCoV,with a detection rate of 1.81%.In conclusion,local piglets were suffered from viral diarrhea mainly in winters and springs in Henan Province,caused by the major viral pathogens including PEDV,PoRV and PDCoV,and mixed infection was available with certain regional characteristics.Local farms and veterinary authorities were supported by the data obtained in the study to develop prevention and control strategies for porcine viral diarrhea.

关 键 词：病毒性腹泻 仔猪 流行特征 河南省 

分 类 号：S851.3[农业科学—预防兽医学]